作物学报2017，Vol.43Issue(1)：19-30,12.DOI:10.3724/SP.J.1006.2017.00019
向日葵高密度遗传连锁图谱构建及两种水分条件下芽期性状的 QTL分析
Construction of High-density Genetic Map and QTL Mapping for Seed Ger-mination Traits in Sunflower under Two Water Conditions
摘要
Abstract
Seed germination and emergence of sunflower are seriously affected by water stress. In this study, SSR, SRAP, and AFLP markers were applied to construct a genetic linkage map by using the F6population derived from a cross of K55 (drought sensitive)´ K58 (drought resistant). For mapping quantitative trait loci (QTLs) for ninetraits of seed germination in sunflower, the parents and 187 F6family lines were used to investigate seed germination traits under normal condition (CK) and 18% polyethylene glycol (PEG-6000) (drought stress). A genetic map consisting of 17 linkage groups was constructed with 1105 loci (368 SSR, 368 SRAP, 369 AFLP) which covers 3846.0 cM and the length of each linkage group varies from 147.6 to 295.5 cM, the number of markers in each linkage group varies from 10 to 165 with an average distance of 3.48 cM. As a result, a total of 33 QTLs were detected. We identified fourteen additive QTLs for germination index (GI), germination rate (GR), embryo length (EL), radicle length (RL), embryo fresh weight (EFW), radicle fresh weight (RFW) under18% PEG con-dition with explained phenotypic variance ranging from 6.1% to 14.0%. Nineteen additive QTLs were identified for germina-tion energy(GE), radicle length (RL), embryo fresh weight (EFW), radicle fresh weight (RFW), embryo dry weight (EDW), radicle dry weight (RDW) under normal condition with explained phenotypic variance ranging from 6.1% to 25.8%. Each of Qefw5-2,Qefw5-1,Qefw5-4,Qrfw10,Qrfw5, andQrl9 could explain phenotypic variance over 10%. Nine QTLs affecting trait differences between stress treatment and control were identified, which are considered to directly contribute to drought toler-ance. These QTLs identified could provide important reference to molecular breeding for drought-resistance during seed ger-mination in sunflower.关键词
向日葵/遗传连锁图谱/耐旱性/芽期性状/QTLKey words
Sunflower/Genetic linkage map/Drought tolerance/Seed germination traits/QTL引用本文复制引用
吕品,于海峰,于志贤,张永虎,张艳芳,王婷婷,侯建华..向日葵高密度遗传连锁图谱构建及两种水分条件下芽期性状的 QTL分析[J].作物学报,2017,43(1):19-30,12.基金项目
本研究由国家自然科学基金项目(31160288,31540043)资助。 The study was supported by the National Natural Science Foundation of China (31160288,31540043) （31160288,31540043）
