作物学报2017，Vol.43Issue(1)：31-41,11.DOI:10.3724/SP.J.1006.2017.00031
甘蔗独脚金内酯生物合成关键基因ScD27的克隆与表达分析
Cloning and Expression Analysis of Key GeneScD27in Strigolactones Biosynthesis Pathway
摘要
Abstract
Strigolactones (SLs) is a novel class of plant hormones. D27 regulating reversible metabolic process is located in up-stream of strigolactones biosynthesis pathway. In this study, primers were designed based on the conserved domains from four species inludingOryza sativa,Zea mays,Sorghum bicolor, andBrachypodium distachyon. Using cDNA from sugarcane cultivar ROC22 as the template, the full-length cDNA sequence ofD27gene from sugarcane was cloned by RT-PCR and RACE method. This gene is named asScD27, with the GenBank accession number of KP987221.1. Its length is 1379 bp, and it contains an 867 bp open reading frame (ORF), encoding 288 amino acid residues. ScD27 is not a secretory protein and has a molecular weight of 71.58 kD, with a theoretical isoelectric point of 5.04. ScD27 is mainly located in chloroplast and the conserved domains of this protein involve two zinc finger protein structures (ZnF_TAZ and ZnF_A20). Amino acid sequences encoded by ScD27shared more than 70% similarity with the reported amino acid sequences encoded byD27 ofSorghum bicolor, Setaria italicaBeauv., Hordeum vulgare subsp. vulgareand Brachypodium distachyon.ScD27 gene was differentially expressed in different parts of sugarcane plant, with higher level of transcript in stem tip and axillary bud but much lower level in leaf, stem and root. Further-more, the expression ofScD27 could be induced by the stresses of PEG, salt and the deficiencies of phosphorus and nutrition. These results demonstrated thatScD27 might be a key gene participating in the response to abiotic stresses during sugarcane SLs biosynthesis pathway.关键词
甘蔗/ScD27/同源克隆/生物信息学/q-PCRKey words
Sugarcane/ScD27/Homology cloning/Bioinformatics/q-PCR引用本文复制引用
吴转娣,赵培方,吴才文,刘新龙,刘家勇,昝逢刚,李旭娟,刘洪博,林秀琴,陈学宽,苏火生..甘蔗独脚金内酯生物合成关键基因ScD27的克隆与表达分析[J].作物学报,2017,43(1):31-41,11.基金项目
本研究由国家自然科学基金项目(31360359),国家现代农业产业技术体系建设专项(CARS-20-1-1),云南省中青年学术技术带头人后备人才(2014HB038),云南省应用基础研究计划项目(2016FB071),重大科技专项-生物(2015ZA001)和科技创新人才计划(2014HC015)资助。This study was supported by the National Natural Science Foundation of China (31360359), the National Modern Agricultural Industry Technology System Construction Project (CARS-20-1-1), the Young and Middle-aged Academic Technology Leaders Reserve Talented Per-son in Yunnan Province (2014HB038), the Applied Basic Research Projects in Yunnan Province (2016FB071), the Major Science and Tech-nology Projects - Biology (2015ZA001), and the Science and Technology Innovation Talents Project (2014HC015) （31360359）
