作物学报2017，Vol.43Issue(1)：42-50,9.DOI:10.3724/SP.J.1006.2017.00042
甘蔗CDK基因的cDNA全长克隆与表达分析
Cloning and Expression Analysis ofCDK Gene in Sugarcane
摘要
Abstract
Plant cyclin-dependent kinases (CDKs) are a specific class of serine/threonine protein kinases, which synergize with cyclins and play an important role in the process of cell regulation. In this study, a unigene that was highly homologous to the Sorghum biocolorCDK sequence (GenBank accession No. XP_002466536.1) was isolated from the sugarcane transcriptome in response toSorghum mosaic virus (SrMV) infection. This gene was validated by RT-PCR and named asScCDK (GenBank acces-sion No. KR258796).ScCDK has a full-length cDNA of 1799 bp and contained a complete open reading frame (65–1603 bp) encoding 512 amino acids. The ScCDK protein contains the typical conservative CDK domains, such as ATP binding site, poly-peptide substrate binding site and activation loop. Bioinformatics prediction indicated that ScCDK is soluble protein located in cell nucleus, very likely involved in intermediary metabolism, and has no signal peptide but with random coil according to its secondary structure. qRT-PCR assay revealed thatScCDK gene had tissue-specific expression in sugarcane, with the highest ex-pression level in bud, followed by stem pith, root, leaf sheath and epidermis. The gene expression level was up-regulated by PEG, NaCl, and ABA, particularly ABA treatment resulted in 1.9-fold higher expression ofScCDK compared to the control. These re-sults suggest thatScCDK participates in the response of drought and osmotic stresses and the cell cycle division in sugarcane and can be induced by ABA.关键词
甘蔗/CDK基因/同源克隆/生物信息学分析/实时荧光定量PCRKey words
Sugarcane/CDK/Homology cloning/Bioinformatics analysis/qRT-PCR引用本文复制引用
苏亚春,阙友雄,黄珑,凌辉,王竹青,刘峰,苏炜华,黄宁,吴期滨,高世武..甘蔗CDK基因的cDNA全长克隆与表达分析[J].作物学报,2017,43(1):42-50,9.基金项目
本研究由福建省杰出青年科学基金(2015J06006),福建省高等学校新世纪优秀人才支持计划(JA14095),引进国际先进农业科学技术计划(948计划)项目(2014-S18)和国家现代农业产业技术体系建设专项(CARS-20)项目资助。 This work was supported by Fujian Natural Science Funds for Distinguished Young Scholar (2015J06006), the Program for New Century Excellent Talents in Fujian Province University (JA14095), the Program of Introducing International Super Agricultural Science and Tech-nology (948 Program)(2014-S18), and the China Agriculture Research System (CARS-20) （2015J06006）
