作物学报2017，Vol.43Issue(1)：63-71,9.DOI:10.3724/SP.J.1006.2017.00063
玉米穗行数主效位点qKRN5.04精细定位与遗传效应解析
Fine Mapping and Genetic Effect Analysis ofqKRN5.04, a Major QTL Associ-ated with Kernel Row Number in Maize
摘要
Abstract
Kernel row number (KRN) is one of the important factors of maize yield. The genetic basis dissection and fine mapping of crucial gene would be greatly beneficial to maize molecular breeding. In present study, series of advanced backcross population and secondary segregation population were developed from a backcross of the derived line of Silunuo (four kernel rows, as the donor parent) and Nong 531 (18-22 kernel rows, as the recurrent parent). The QTL mapping for KRN was conducted by the in-clusive complete interval mapping (ICIM) method in multiple environments. And a major KRN QTL,qKRN5.04, was mapped to the interval of 136.3-140.0 Mb on chromosome 5, with the largest phenotypic variation of 21.76% and the effect value of 0.80-1.76 row. Furthermore, according to recombinant analysis of secondary population,qKRN5.04 was fine mapped to the re-gion of about 300 kb, which provided both practical InDel markers for marker-assisted selection and sufficient supports for the map-based cloning and candidate gene mining of the target locus.关键词
玉米/穗行数/QTL/精细定位Key words
Maize/Kernel row number (KRN)/Quantitative trait locus (QTL)/Fine mapping引用本文复制引用
白娜,石云素,李永祥,焦付超,陈林,李春辉,张登峰,宋燕春,王天宇,黎裕..玉米穗行数主效位点qKRN5.04精细定位与遗传效应解析[J].作物学报,2017,43(1):63-71,9.基金项目
本研究由国家公益性行业(农业)科研专项,作物种质资源保护与利用专项(201303007,2015NWB030-04),国家科技支撑计划项目(2013BAD01B02-3),国家自然科学基金项目(91335206)和中国农业科学院科技创新工程项目资助。 This study was supported by the Special Fund for Agro-scientific Research in the Public Interest, the Special Fund for Protection and Utilization of Crop Germplasm Resources (201303007,2015NWB030-04), the National Key Technology Support Program of China (2013BAD01B02-3), the Na-tional Natural Science Foundation of China (91335206), and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences （农业）
