作物学报2017，Vol.43Issue(1)：141-148,8.DOI:10.3724/SP.J.1006.2017.00141
外源ABA对低温胁迫下玉米幼苗内源激素含量及Asr1基因表达的调节
Regulation on Contents of Endogenous Hormones andAsr1 Gene Expression of Maize Seedling by Exogenous ABA under Low-temperature Stress
摘要
Abstract
Abscisic acid (ABA) is an important signal factor under low-temperature stress. This study aimed to investigate the effect of exogenous ABA on growth regulation of maize seedlings under low-temperature stress. Maize variety “Jiulong 5” resis-tant to low temperature was used with foliage spray of ABA (5, 15, 25, 35 mg L–1) at three-leaf period under varying degrees of low-temperature, to analyze relative electric conductivity, activities of autioxidant enzymes and contents variation of endogenous ABA and IAA of maize blades, as well asAsr1 gene expression level by Real-time PCR. The relative electric conductivity of maize blades showed an increasing tendency, meanwhile, activities of SOD and POD were enhanced by exogenous ABA treat-ments among which SOD activity was significantly higher in ABA treatments of 15 mg L–1 and 25 mg L–1than in the control un-der low-temperature stress. Also the synthesis of endogenous ABA and IAA were increased significantly. The expression level of Asr1 gene significantly promoted in ABA treatments of 5, 15, and 25 mg L–1. ABA contant had a highly significant correlation with the relative expression ofAsr1 gene and SOD activity and a significant correlation with POD activity. In conclusion the ex-pression ofAsr1 gene is mediated and regulated by ABA, the promotion ofAsr1 gene expression also promotes the synthesis of endogenous ABA, and enhances antioxidant enzyme activities, resulting in improved maize resistance to low temperature. But the mediation and regulation of exogenous ABA have a concentration effect, showing promotion at low concentation and inhibition at high concentation.关键词
玉米/外源ABA/Asr1基因/低温胁迫/内源激素Key words
Maize/Exogenous ABA/Asr1gene/Low-temperature stress/Endogenous hormone引用本文复制引用
李馨园,杨晔,张丽芳,左师宇,李丽杰,焦健,李晶..外源ABA对低温胁迫下玉米幼苗内源激素含量及Asr1基因表达的调节[J].作物学报,2017,43(1):141-148,8.基金项目
本研究由国家自然科学基金项目(31401320)资助。 This study was supported by the National Natural Science Foundation of China (31401320) （31401320）
