北京林业大学学报2016，Vol.38Issue(12)：64-72,9.DOI:10.13332/j.1000-1522.20150404
长白落叶松小RNA测序和其靶基因预测
Small RNA sequencing and target gene prediction in Larix olgensis
摘要
Abstract
miRNA is a relatively special and highly conserved RNA, which plays an important role in gene regulation network. In this study, with the help of Illumia sequencing platform and the Solexa sequencing analysis of miRNA in Larix olgensis, we ultimately selected 15 294 797 clean reads, in which rRNA is 2 991 972 reads, tRNA 65 264 reads, snRNA 1 670 reads and snoRNA 467 reads, and ncRNAs and repeat sequences contain 6 421 reads. Un-annotated miRNA has 12 229 003 reads, accounting for 79. 96% of the total RNA notes. The miRDeep2 was used to screen the miRNA, and 78 new miRNAs were obtained overall, which belong to the miR164, miR166, miR950, miR160, and miR396 families. Through further analysis of the target gene notes, we obtained 333 corresponding target genes, and their functions include transcription factors, unknown functional proteins, binding proteins, elongation factors, signal transduction, cell wall and cell membrane biosynthesis involved in regulation of plant growth and development process.关键词
小RNA/长白落叶松/miRNA/靶基因Key words
sRNA/Larix olgensis/miRNA/target gene分类
农业科技引用本文复制引用
张素芳,张磊,赵佳丽,张莉,张含国..长白落叶松小RNA测序和其靶基因预测[J].北京林业大学学报,2016,38(12):64-72,9.基金项目
“863”国家高技术研究发展计划项目(2013AA102704)、中央高校基本科研业务费专项(2572016CA08、2572016AA36)。 （2013AA102704）
