川北医学院学报2016，Vol.31Issue(6)：782-785,4.DOI:10.3969/j.issn.1005-3697.2016.06.001
犬骨髓间充质干细胞体外分离培养及分化鉴定
Isolation,culture,differentiation and identification of dog mesenehymal stem cells in vivo
摘要
Abstract
Objective:To establish a method for isolation,culture,differentiation and identification of bone mallow mesenchymal stem cells(BMSCs)from dog.Methods:dBMSCs were isolated by combination of gradient centrifugation and adherent wall culture,and induced their differentiation into osteoblasts and adipocytes which were identified by histoehemistry staining and oil red staining.Re-sults:The cultured adherent cells showed spindle and polygonal shape.The identification of dBMSCs was carried out by alizarin red stai-ning after induction of osteoblasts and alkaline phosphatase staining after induction of adipocytes.Conclusion:dBMSCs were isolated and cultured successfully from dog ilium bone marrow by combination of gradient centrifugation and adherent wallculture,which possess multi-directional differentiation potential,is the ideal seed cells in tissue engineering research.关键词
组织工程/骨髓间充质干细胞/分离/培养/诱导/分化Key words
Tissue engineering/Bone mesenehymal stem cells/Isolation/Culture/Induce/Differentiation分类
医药卫生引用本文复制引用
蒲超,张珊珊,李伟,吴青霞,吴辉..犬骨髓间充质干细胞体外分离培养及分化鉴定[J].川北医学院学报,2016,31(6):782-785,4.基金项目
川北医学院科研发展计划项目 （）
