重庆医学2017，Vol.46Issue(1)：90-93,4.DOI:10.3969/j.issn.1671-8348.2017.01.021
两种重编程系统诱导人牙源性多潜能干细胞的对比研究
A comparative study of two reprogramming systems for inducing pluripotent stem cells from human dental origin
摘要
Abstract
Objective To comparatively study the features of two reprogramming systems of induced pluripotent stem cells (iPSCs)from human dental origin.Methods Two kinds of reprogramming system,i.e.STEMCCA lentivirus /feed layer and Sen-dai virus /matrigel were used to induce human stem cells from apical papilla(SCAP)into iPSCs,respectively.The induction efficien-cies,workload of generating iPSCs,aneuploidy karyotype ratio,complexities of eliminating exogenous transcription factors and spe-cific markers expression were compared between these two systems.Results The STEMCCA reprogramming system required to prepare the feeder cell MEF.The reprogramming efficiency was 0.1%.Transcription gene-free iPSCs cells were obtained by the Cre-loxp enzyme digestion technique at the later stage.Sendai virus reprogramming system was feeder-free and the preparation of matrigel was quite simple with unified standard.The reprogramming efficiency was 0.7%,which was much higher than that of STEMCCA system(P <0.05).The exogenous virus and transgenes could be gradually eliminated after several passages of natural subclone.Conclusion The Sendai virus/matrigle reprogramming system is much more applicable for the induction of iPSCs from dental origin than the STEMCCA system.关键词
多潜能干细胞/乳头/干细胞/慢病毒属/人诱导性多潜能干细胞/根尖乳头干细胞/重编程/仙台病毒Key words
pluripotent stem cells/nipples/stem cells/lentivirus/human induced pluripotent stem cells/stem cells from apical papilla/reprogramming/sendai virus分类
医药卫生引用本文复制引用
谭小兵,徐静舒,孙贵虎,宋俊呈,戴青原..两种重编程系统诱导人牙源性多潜能干细胞的对比研究[J].重庆医学,2017,46(1):90-93,4.基金项目
国家自然科学基金资助项目（81360161）；云南省教育厅基金资助项目（2015Y153）。 （）
