动物营养学报2017，Vol.29Issue(1)：60-68,9.DOI:10.3969/j.issn.1006-267x.2017.01.008
相对湿度对递增性偏热环境下肉鸡体热调节及下丘脑热休克蛋白70含量的影响
Effects of Relative Humidity at Gradually Increasing Temperatures on Body Thermoregulation and Content of Heat Shock Protein 70 in Hypothalamus of Broiler Chickens
摘要
Abstract
The present study aimed to investigate the effects of relative humidity ( RH) at gradually increasing temperatures on body thermoregulation and the content of heat shock protein 70 ( HSP70) in hypothalamus of broiler chickens. One hundred and eighty 21⁃day⁃old Arbor Acres ( AA) broiler chickens were allotted into en⁃vironment controlled chambers and randomly assigned to three groups with six replicates per group and ten broilers per replicate ( males and females in half) . The pre⁃test period lasted for 7 days and birds were kept at 20 ℃ and 60% RH. The test period lasted for 15 days, when broiler chickens were 28 days of age, RH of three groups was regulated to 35%, 60% and 85%, respectively, and the temperature was 20 ℃ yet. The ex⁃periment consisted of 5 consecutive trials with 3 days per consecutive trial, the temperature of three groups was gradually increased by 3 ℃ finishing within half an hour at 10:00 of the first day every consecutive trial and started from the second consecutive trial, and the temperatures of the 5 consecutive trials were 20, 23, 26, 29 and 32 ℃, respectively. The results showed as follows:1) the respiratory frequency of broiler chickens in the 85% RH group was significantly higher than that in the 60% RH and 35% RH groups ( P<0.01) , and the re⁃spiratory frequency in the 60% RH group was significantly higher than that in the 35% RH group ( P<0.01) . The core body temperature of broiler chickens in the 85% RH group was significantly higher than that in the 60% RH group ( P<0.05) . The skin temperatures of leg and flipper of broiler chickens in the 35% RH group were significantly higher than those in the 60% RH and 85% RH groups ( P<0.01) , and the skin temperatures of comb, earlobe and eyelid in the 35% RH and 60% RH groups were significantly higher than those in the 85% RH group ( P<0.01) . 2) The activity of alkaline phosphatase ( AKP) in serum of broiler chickens in the 85% RH group was significantly higher than that in the 60% RH and 35% RH groups ( P<0.05) , and the ac⁃tivity of creatine kinase ( CK) in serum of broiler chickens in the 85% RH and 35% RH groups was signifi⁃cantly higher than that in the 60% RH group ( P<0.05) . There were no significant effects of RH at gradually increasing temperatures on the activities aspartate transaminase ( AST ) and lactate dehydrogenase ( LDH ) in serum of broiler chickens (P>0.05). 3) The content of 3,5,3’⁃triiodothyronine (T3) in serum of broiler chickens in the 85% RH group was significantly higher than that in the 35% RH group ( P<0.05) , the content of 3,5,3’,5’⁃tetraiodothyronine (T4) in serum in the 35% RH and 85% RH groups was significantly higher than that in the 60% RH group ( P<0.05) , and the content of cortisol ( CORT) in serum in the 60% RH and 85% RH groups was significantly higher than that in the 35% RH group ( P<0.01) . 4) The content of HSP70 in hypothalamus of broiler chickens in the 35% RH group was significantly lower than that in the 60% RH and 85% RH groups ( P<0.05) . In conclusion, at the 15⁃day 20 to 32 ℃ gradually increasing temperatures, high humidity ( 85%) and low humidity ( 35%) regulate the body heat balance of broiler chickens at 28 to 42 days of age in different ways and extent.关键词
递增性偏热环境/肉仔鸡/相对湿度/体热调节/热休克蛋白70Key words
gradually increasing temperatures/broiler chickens/relative humidity/body thermoregulation/heat shock protein 70分类
农业科技引用本文复制引用
周莹,张敏红,冯京海,张少帅,彭骞骞,李萌,李香..相对湿度对递增性偏热环境下肉鸡体热调节及下丘脑热休克蛋白70含量的影响[J].动物营养学报,2017,29(1):60-68,9.基金项目
国家“十二五”科技支撑课题（2012BAD39B02）；国家重点研发计划课题“肉禽舒适环境的适宜参数及限值研究”（2016YFD0500509）；中国农业科学院科技创新工程 （）
