动物营养学报2017，Vol.29Issue(1)：290-297,8.DOI:10.3969/j.issn.1006-267x.2017.01.033
艾蒿水提物对肉仔鸡血清细胞因子含量及小肠诱导型一氧化氮合成酶mRNA表达量的影响
Effects of Artemisia argyi Aqueous Extract on Serum Cytokines Contents and Small Intestine Inducible Nitric Oxide Synthase mRNA Expression Level of Broilers
摘要
Abstract
This experiment was conducted to study the effects of dietary different supplementation of Artemisia argyi aqueous extract ( AAE ) on serum cytokines contents and small intestine inducible nitric oxide synthase ( iNOS) mRNA expression level of broilers. A total of 192 one⁃day⁃old Arbor Acre ( AA) broilers were ran⁃domly divided into four groups with six replicates per group and eight chickens per replicate. Broilers in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diet supple⁃mented with 500, 1 000 and 2 000 mg/kg AAE, respectively. The experiment lasted for 42 days. The results showed as follows:1) compared with the control group, at 21 days of age, the contents of interleukin⁃1β( IL⁃1β) , interleukin⁃2 ( IL⁃2) and interleukin⁃4 ( IL⁃4) in serum of 2 000 mg/kg supplementation group were significantly improved ( P<0.05) , and the serum IL⁃4 content of 500 mg/kg supplementation group and the serum IL⁃2 content of 1 000 mg/kg supplementation group were significantly improved ( P<0.01); at 42 days of age, the serum IL⁃2 content of 500 mg/kg supplementation group was significantly increased ( P<0.05) . 2) Compared with the control group, at 21 days of age, the nitric oxide ( NO) content and iNOS ac⁃tivity in serum and iNOS mRNA expression level in duodenum, jejunum and ileum of 500 mg/kg supplementa⁃tion group had no significant difference ( P>0.05); the serum NO content and the ileum iNOS mRNA expres⁃sion level of 1 000 mg/kg supplementation group were significantly increased ( P<0.05);the ileum iNOS mR⁃NA expression level of 2 000 mg/kg supplementation group was significantly increased ( P<0.01);at 42 days of age, the NO content and iNOS activity in serum and the jejunum iNOS mRNA expression level of 500, 1 000 and 2 000 mg/kg supplementation groups were significantly improved ( P<0.05 or P<0.01) . The results suggest that dietary supplementation of AAE can improve the secretion of cytokines and the production of NO, and it is related to the expression enhancement of iNOS mRNA.关键词
艾蒿水提物/肉仔鸡/细胞因子/诱导型一氧化氮合成酶/mRNAKey words
Artemisia argyi aqueous extract/broilers/cytokines/inducible nitric oxide synthase/mRNA分类
农业科技引用本文复制引用
赵飞,史彬林,陈宏燕,佟满满,孙登生,张鹏飞,金晓,闫素梅..艾蒿水提物对肉仔鸡血清细胞因子含量及小肠诱导型一氧化氮合成酶mRNA表达量的影响[J].动物营养学报,2017,29(1):290-297,8.基金项目
国家自然科学基金资助项目 （）
