南方医科大学学报2017，Vol.37Issue(1)：6-12,7.DOI:10.3969/j.issn.1673-4254.2017.01.02
脱氧胆酸对C57BL/6小鼠回肠类器官生长的影响
Effect of deoxycholic acid intervention on growth of ileum organoids derived from C57BL/6 mice
摘要
Abstract
Objective To establish a culture system for mouse intestinal organoids and investigate the effect of deoxycholic acid (DCA) on organoids growth. Methods The terminal ileum was collected from 8-month-old C57BL/6 mice. The tissue blocks were treated with EDTA and the crypts were collected and embedded in Matrigel Matrix. Orgnoids growth and buddings were observed in the control group, anhydrous alcohol group, short-term (2 days) 100 μmol/L DCA treatment group, and long-term (10 days) 10 μmol/L DCA treatment group; the orgnoids were further cultured for 10 days after removal of DCA from the medium and observed for orgnoids growth and buddings. Results Short-term treatment with high-concentration DCA resulted in significantly reduced enterosphere formation, enteroids formation, progression from enterospheres to enteroids and number of crypt buds per enteroid (P<0.05), which remained unchanged even after removal of DCA for a short time (P<0.05);long after DCA removal, the enteroids formation rate and number of the crypt buds still remained lower than those in normal organoids (P<0.05). Short-term treatment with low-concentration DCA only resulted in reduced enteroids formation rate and number of crypt buds (P<0.05), and prolonged treatment caused reduced enterospheres formation rate, enteroids formation rate and number of crypt buds (P<0.05). After DCA removal, enteroids formation rate and the number of crypt buds still remained lower than those in the normal group (P<0.05). Conclusion We successfully established an organoids culture system. The presence of DCA in the culture system affects the growth of the organoids, which can partly recover following a prolonged period after the removal of DCA.关键词
类器官/隐窝/脱氧胆酸/小肠干细胞Key words
organoids/crypts/deoxycholic acid/intestinal stem cells引用本文复制引用
楼俪泓,曾悦,周慧,陆颖影,王兴鹏..脱氧胆酸对C57BL/6小鼠回肠类器官生长的影响[J].南方医科大学学报,2017,37(1):6-12,7.基金项目
上海交通大学医工交叉基金（YG2014ZD10）；上海交通大学医工交叉基金（YG2015MS29）；上海市胰腺疾病重点实验室开放课题 （）
