南方医科大学学报2017，Vol.37Issue(1)：24-29,6.DOI:10.3969/j.issn.1673-4254.2017.01.05
鼠李糖乳杆菌培养上清液通过抑制NF-κB通路预防大肠杆菌性脑膜炎
Lactobacillus rhamnosus GG conditioned medium prevents E. coli meningitis by inhibiting nuclear factor-κB pathway
摘要
Abstract
Objective To investigate whether Lactobacillus rhamnosus GG conditioned medium(LGG-CM)has preventive effect against E. coli K1-induced neuropathogenicity in vitro by inhibiting nuclear factor-κB (NF-κB) signaling pathway. Methods An in vitro blood-brain barrier (BBB) model was constructed using human brain microvascular endothelial cells (HBMECs). The effect of LGG-CM on E. coli-actived NF-κB signaling pathway was assayed using Western blotting. Invasion assay and polymorphonuclear leukocyte (PMN) transmigration assay were performed to explore whether LGG-CM could inhibit E. coli invasion and PMN transmigration across the BBB in vitro. The expressions of ZO-1 and CD44 were detected using Western blotting and immunofluorescence. The changes of trans-epithelial electric resistance (TEER) and bacterial translocation were determined to evaluate the BBB permeability. Results Pre-treament with LGG-CM inhibited E. coli-activated NF-κB signaling pathway in HBMECs and decreased the invasion of E. coli K1 and transmigration of PMN. Western blotting showed that LGG-CM could alleviate E. coli-induced up-regulation of CD44 and down-regulation of ZO-1 expressions in HBMECs. In addition, pre-treatment with LGG-CM alleviated E. coli K1-induced reduction of TEER and suppressed bacterial translocation across the BBB in vitro. Conclusion LGG-CM can block E. coli-induced activation of NF-κB signaling pathway and thereby prevents E. coli K1-induced neuropathogenicity by decreasing E. coli K1 invasion rates and PMN transmigration.关键词
鼠李糖乳杆菌/上清液/脑膜炎/人脑血管内皮细胞/E. coli K1/NF-κBKey words
Lactobacillus rhamnosus GG/conditioned medium/meningitis/human brain microvascular endothelial cells/E. coli K1/nuclear factor-κB引用本文复制引用
曾庆,何肖龙,肖汉森,杜蕾,李雨静,陈乐程,田慧文,黄胜和,曹虹..鼠李糖乳杆菌培养上清液通过抑制NF-κB通路预防大肠杆菌性脑膜炎[J].南方医科大学学报,2017,37(1):24-29,6.基金项目
国家自然科学基金（81370740）；南方医科大学公卫学院院长基金（GW201601） Supported by National Natural Science Foundation of China (81370740) （81370740）
