南方医科大学学报2017，Vol.37Issue(1)：30-35,6.DOI:10.3969/j.issn.1673-4254.2017.01.06
SD大鼠外阴慢性单纯性苔藓模型的构建及蛋白酶激活受体2的表达
Establishment of a SD rat model of vulvar lichen simplex chronicus and detection of the expression of protease activated receptor 2
摘要
Abstract
Objective To establish a SD rat model of vulvar lichen simplex chronicus (LSC) and investigate the expression of protease activated receptor 2 (PAR2) in the genital skin. Methods Seventy female SD rats were randomly divided into group A (blank control group, n=10), group B (with application of acetone solution 3 times per week for 10 weeks, n=10), group C (with chronic mechanical irritation 3 times per week for 10 weeks, n=10), and group D (with topical treatment with 0.5% 7, 12-Dimethylbenzanthracene [DMBA] in acetone solution and chronic mechanical irritation 3 times per week for 10 weeks, n=40). The changes of the genital skin changes were observed regularly and the expression of PAR2 in groups A and D was detected with immunohistochemistry, Western blotting and qRT-PCR. Results In group D, LSC occurred in 23 rats (57.5%) at 8 weeks and in 38 rats (95%) at 10 weeks;8 rats (20%) showed papilloma at 12 weeks. Acetone treatment or chronic mechanical irritation did not cause LSC in the rats. Immunohistochemistry, Western blotting and qRT-PCR showed significantly increased expressions of PAR2 in group D at both the protein and mRNA levels as compared with those in group A (P<0.05). Conclusion 0.5%DMBA in acetone solution along with chronic mechanical irritation can induce LSC in female SD rats, and PAR2 is closely related with the occurrence and progression of LSC.关键词
外阴慢性单纯性苔藓/动物模型/蛋白酶激活受体2Key words
vulvar lichen simplex chronicus/animal model/protease activated receptor 2引用本文复制引用
杨欢,付贞花,唐华均,李成志..SD大鼠外阴慢性单纯性苔藓模型的构建及蛋白酶激活受体2的表达[J].南方医科大学学报,2017,37(1):30-35,6.基金项目
十二五科技支撑计划 （）
