南方医科大学学报2017，Vol.37Issue(1)：36-43,8.DOI:10.3969/j.issn.1673-4254.2017.01.07
维格列汀通过上调胰高血糖素样肽1发挥抗癫痫作用
Vildagliptin suppresses temporal lobe epilepsy by up-regulating glucagon-like peptide-1
摘要
Abstract
Objective To investigate the effect of vildagliptin on pentamethazol (PTZ)-induced epilepsy in rats and explore the molecular mechanism. Methods Samples of temporal cortex from 23 patients with temporal lobe epilepsy were collected as epilepsy group and samples of temporal cortex from 14 patients with brain trauma were used as control group. Ninety male SD rats were randomly divided into control group (group A), PTZ-induced epilepsy group (group B), saline 2 mL/kg group (group C), vildagliptin 2.5 mg/kg group (group D), vildagliptin 5mg/kg group (group D) and vildagliptin 10 mg/kg group (group F). Use chronic model of epilepsy induced by PTZ (35 mg/kg) intraperitoneal injection for 3 consecutive weeks, and changes of behavior were observed. The expression of GLP-1R was detected by Western blotting and immunohistochemical (IHC) staining, and the expression of GLP-1 was detected by enzyme-linked immunosorbent assay (ELISA). The location of GLP-1R was detected by immunofluorescent staining. Results Immunofluorescent staining showed that the GLP-1R located in the neurons, and GLP-1R expression was obviously decreased both in patients with TLE and in rats with epilepsy. The latency time was prolonged and epilepsy attack time was decreased after vildagliptin treatment (P<0.05). GLP-1R expression was increased after vildagliptin treatment (P<0.05). ELISA showed the change of GLP-1 expression was the same as GLP-1R. Conclusion Vildagliptin can suppress temporal lobe epilepsy in rats by up-regulating GLP-1 and GLP-1R expressions.关键词
维格列汀/颞叶癫痫/胰高血糖素样肽1受体/胰高血糖素样肽1Key words
vildagliptin/temporal lobe epilepsy/glucagon-like peptide-1 receptor/glucagon-like peptide-1引用本文复制引用
温跃桃,吴昆仑,石全红..维格列汀通过上调胰高血糖素样肽1发挥抗癫痫作用[J].南方医科大学学报,2017,37(1):36-43,8.基金项目
重庆市卫生局重点资助项目 （）
