森林与环境学报2017，Vol.37Issue(1)：47-53,7.DOI:10.13324/j.cnki.jfcf.2017.01.008
杉木种子多酚提取工艺优化
Optimization of ultrasonic-assisted extraction process for polyphenols from the seeds of Chinese fir
摘要
Abstract
In order to evaluate the variation of polyphenols in the seeds of different families of Chinese fir at different growth phases, and offer technical support for elucidating the underlying mechanisms of the abortive seeds formation in Chinese fir. An ultrasonic-assisted extraction method was applied to the effective extraction of polyphenols from the abortive and healthy seeds of Chinese fir. The effects of extraction times, extraction duration, ethanol concentration, extraction temperature and solvent-to-sample ratio on the yield of polyphenols from the seeds of Chinese fir were observed, respectively. Based on the results of single-factor experiments, an orthogonal design was employed to further optimize the extraction process. The results showed that the optimal extraction conditions for abortive seeds were 50% ethanol concentration, the solvent-to-sample ratio was 60 ∶ 1, and extraction for one time at 50 ℃ for 15 min. Under these optimal conditions, the maximum yield of polyphenols from abortive seeds was (383.22±1.23) mg·g-1. At the same time, the optimal extraction conditions for healthy seeds were 100% ethanol concentration, the solvent-to-sample ratio was 25∶ 1, and extraction for one time at 50℃ for 25 min. Under these optimal conditions, the maximum yield of polyphenols from healthy seeds was (18.360±0.069) mg·g-1. Verification test showed that the optimized extraction parameters were stable and reliable.关键词
涩粒/健籽/多酚提取量Key words
abortive seeds/healthy seeds/the yield of polyphenols分类
农业科技引用本文复制引用
林小琴,马志慧,何宗明,陈宇,彭亦如,林思祖..杉木种子多酚提取工艺优化[J].森林与环境学报,2017,37(1):47-53,7.基金项目
国家林业局杉木工程技术研究中心平台建设( ptjh130002) （ ptjh130002）
福建农林大学校重点项目建设专项“人工林培育学科建设”(6112c0310) （6112c0310）
林学学科建设项目(6112c0300)。 （6112c0300）
