福建农林大学学报（自然科学版）2017，Vol.46Issue(1)：109-114,6.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2017.01.017
一种通用高效液相色谱测定植物叶片内源激素水杨酸的方法
A general method for detecting the content of endogenous salicylic acid in plant leaf by HPLC
摘要
Abstract
To accurately determine salicylic acid ( SA) level in plant, methanol was used to extract SA from Arabidopsis leaf, with 3-hydroxy benzoic acid being the internal standard. After acid hydrolysis, the hydrolysate was partitioned with ethyl acetate and hexane mixture (1 : 1), and followed by high performance liquid chromatography (HPLC) analysis under different ratios and pH of the mobile phase. Results showed that SA was thoroughly separated from the complex mixture when methanol accounted for 10% of sodi-um acetate and methanol mixture under pH 6.0. Furthermore, rice and narcissus leaf was used to verify this method, whose results confirmed that this method was generally applicable for SA detection in plants.关键词
水杨酸/高效液相色谱/3-HBA/植物叶片Key words
salicylic acid/high performance liquid chromatography/3-hydroxy benzoic acid/plant leaf分类
农业科技引用本文复制引用
林文芳,张虹,洪庄玉,缪颖,林文雄..一种通用高效液相色谱测定植物叶片内源激素水杨酸的方法[J].福建农林大学学报（自然科学版）,2017,46(1):109-114,6.基金项目
福建省自然科学基金项目(2016J01103). （2016J01103）
