福建农业学报2016，Vol.31Issue(11)：1164-1170,7.DOI:10.19303/j.issn.1008-0384.2016.11.005
花生长链酰基辅酶 A 合成酶6基因（LACS 6）的克隆、鉴定与组织表达
Cloning,Verification and Tissue Expression of Long-chain Acyl-CoA Synthetase 6 (LACS 6) Gene in Peanut (Arachis hypogaea L.)
摘要
Abstract
Long-chain acyl coenzyme A synthetase (LACS)is one of the important catalytic lipid metabolic enzymes in peanut plants.To understand the mechanism of the fatty acid metabolism in peanut (Arachis hypogaea L.),the cDNA of LACS 6 gene was cloned by RT-PCR (GenBank accession number:KU301860 ).Its structure was analyzed,homology with other plants compared,and mRNA expression in different tissues obtained using real-time PCR.The results showed that the cDNA was 2,1 1 6 bp including an open reading frame of 2,088 bp,encoded 695 amino acids,and had 23 exons and 22 introns.Its amino acid sequence alignment showed a conserved domain of eukaryotic long-chain fatty acid CoA synthetase,as well as conserved activation and binding sites.The homologies of LACS 6 gene with the genes from Cicer arietinum ,Vigna radiate ,Glycine max ,Prunus mume and others were between 79% and 87%.The evolutionary tree indicated that the gene was close to those from leguminous plants, such as chickpeas.The real-time PCR analysis showed that it was differentially expressed in the ovary stalks,roots, stems,leaves,flowers and kernels on a peanut plant.The ovary stalks and flowers had the highest expressionamong all,and with significant differences,The order of expression ranked as flowers > ovary stalks > leaves >kernels > stems > roots.The information obtained would benefit further studies on the fatty acid metabolism and quality improvement for peanut.关键词
花生/长链酰基辅酶 A 合成酶/组织表达/油脂Key words
peanut (Arachis hypogaea L.)/long-chain acyl CoA synthetase/tissue expression/lipid分类
农业科技引用本文复制引用
徐日荣,陈湘瑜,唐兆秀..花生长链酰基辅酶 A 合成酶6基因（LACS 6）的克隆、鉴定与组织表达[J].福建农业学报,2016,31(11):1164-1170,7.基金项目
福建省科技计划项目---省属公益类科研院所基本科研专项(2014R1026-4) （2014R1026-4）
