福建农业学报2016,Vol.31Issue(11):1193-1197,5.DOI:10.19303/j.issn.1008-0384.2016.11.010
杂交兰种质资源遗传多样性的 SRAP 分析
SRAP-marker Based Genetic Diversity of Germplasms of Cymbidium Hybrids
摘要
Abstract
SRAP was applied for the determination of the genetic diversity of 24 hybrid cultivars of Cymbidium. Eleven polymorphic primer combinations were screened to result in 1 1 9 amplified DNA bands.Of the amplified DNA,102 were polymorphic,at a ratio of 86.1%.The average number of polymorphic loci amplified by each primer was 9.3. The genetic similarity among the 24 germplasms ranged from 0.43 to 0.98,averaging 0.67. The UPGMA cluster analysis classified the germplasms with a similarity coefficient of 0.69 into 5 groups clearly showing the genetic diversity and relationship among the hybrids.关键词
杂交兰/SRAP/遗传多样性/聚类分析Key words
Cymbidium hybrid/SRAP/genetic diversity/cluster analysis分类
农业科技引用本文复制引用
钟淮钦,林榕燕,黄敏玲,林兵,陈南川..杂交兰种质资源遗传多样性的 SRAP 分析[J].福建农业学报,2016,31(11):1193-1197,5.基金项目
福建省科技计划项目---省属公益类科研院所基本科研专项(2014R1025-5) (2014R1025-5)
福建省农业科学院科技创新团队 PI 项目(2016PI-39) (2016PI-39)
福建省科技计划项目---星火计划重点项目(2014S0014) (2014S0014)