广西植物2017，Vol.37Issue(1)：29-35,7.DOI:10.11931/guihaia.gxzw201512016
走马胎种质资源遗传多样性的ISSR分析
Efficiency of ISSR markers in assessing genetic diversity and relationships in Ardisia gigantifolia germplasm
摘要
Abstract
Ardisia gigantifolia is a medicinal plant that belongs to genus Ardisia. This plant has been an important tra￣ditional Chinese medicine in China for thousands of years. Now, with the development of pharmacological research, A. gigantifolia is not only used as a clinical medicine, but also plays a role in diet and health aspects. The pharmaco￣logical validity of its medicinal value has extended in an enlarged market. At present, the wild A. gigantifolia in our country is rare, and the cultivars become the new important source. However, the quality and efficacy of cultivars often suffer from intraspecific variation, unstable quality and other issues. Moreover, the cultivars were also affected by ori￣gin, age and environmental conditions. In order to efficiently utilize and conserve this medicinal plant resource, ISSR analysis was employed to reveal genetic diversity of 36 A. gigantifolia germplasm which were collected from different places of Guangxi Zhuang Autonomous Region in China. Data were analyzed by POPGENE32, and a cluster diagram was presented by UPGMA. The results indicated that fourteen ISSR primers generated a total of 136 bands among which 112 (82.35%) were polymorphic bands. Nei’s genetic diversity index (H) was 0.296 5, Shannon diversity in￣dex ( I) was 0.441 7, and the coefficient of gene differentiation ( Gst) was 0.855 8. The genetic similarity coefficient among the populations ranged from 0.667 8 to 0.838 2 in an average of 0.739 1. Based on the clustering analysis, all accessions were clustered into five groups with UPGMA method. Most of the accessions from the same or adjacent re￣gions were clustered into the same group or subgroups. A few accessions, however, were greatly different from the ma￣jority of all accessions. The results suggested that ISSR marker was an effective tool for the study of genetic variation in Chinese natural A. gigantifolia germplasm resources. These results can also be used for germplasm characterization and plant breeding.关键词
走马胎/分子标记/ISSR分子检测/种质资源/遗传多样性Key words
Ardisia gigantifolia/molecular marker/ISSR analysis/germplasm resources/genetic diversity分类
农业科技引用本文复制引用
毛世忠,李景剑,蒋小华,丁莉,赵博,邓涛,马虎生..走马胎种质资源遗传多样性的ISSR分析[J].广西植物,2017,37(1):29-35,7.基金项目
中国科学院“西部之光”人才培养计划项目[科发人教字(2012)179号]；广西自然科学基金[2013GXNSFAA019062]；广西植物研究所基本业务费专项[桂植业13016][Suppoted by West Light Foundation of the Chinese Academy of Sciences [(2012)179]；Guangxi Natural Science Foundation (2013GXNSFAA019062)；Foundamental Science Rescarch Foundation of Guangxi Institute of Botany (13016)]。 （2012）
