广西植物2017，Vol.37Issue(1)：42-48,63,8.DOI:10.11931/guihaia.gxzw201503016
基于ISSR标记的福建省钩锥遗传多样性分析
Genetic diversity analysis on five populations of Castanopsis tibetana from Fujian Province based on ISSR marker
摘要
Abstract
Castanopsis tibetana is an evergreen hard wood tree of red Castanopsis, which is one of the excellent commer￣cial tree species. C. tibetana has great development prospect and ecological value in China. In this paper, the genetic di￣versity of 61 individuals of C. tibetana collected from five plots (Shaowu, Jianyang, Jianou, Zhouning and Pingnan) in Fujian Province of China was analyzed by means of inter￣simple sequence repeat( ISSR) marker, and the genetic rela￣tionship among these individuals also was discussed by cluster analysis method. The results showed that 158 bands were amplified from genomic DNA of 61 C. tibetana individuals by ten inter￣simple sequence repeat ( ISSR) primers, and there were 145 polymorphic bands with percentage of polymorphic band of 91.77%, in which, the percentage of polymor￣phic band of UBC817, UBC819 and UBC42 reached 100.0%. Percentage of polymorphic band(PPB), effective number of alleles ( Ne) , Nei’ s gene diversity ( H) and Shannon’ s diversity index ( I) were obviously different among five C. ti￣betana populations,in which all indexes of population in Shaowu were the highest, populations in Zhouning was the lowest. Gene differentiation coefficient and gene flow of five populations was 0.144 0 and 2.973 0, respectively, indicating that 14.40% of overall genetic variation of five populations exists among populations and 85.60%within popu￣lations. The gene diversity among species ( Ht) and within species ( Hs) were 0.395 8 and 0.338 8. It is suggested that the genetic diversity among species of C. tibetana was relatively rich and the interspecies variation degree was higher than intraspecies. Genetic distance among all populations differed obviously, and the genetic distance, between populations of Shaowu and Jianou was the nearest ( only 0.081 5) while that between populations of Jianyang and Zhouning was the far￣thest (0.162 9). Five populations could be divided into three groups by cluster analysis, in which populations of Pingnan and Zhouning were two separate groups,while three populations of Shaowu,Jianou and Jianyang were gathered into one group, which could be divided into two sub￣groups further, one was Jianyang population and another contained popula￣tions of Shaowu and Jianou. It is suggested that populations of C. tibetana tested possessed higher genetic diversity, and there were frequent gene exchange. The genetic diversity of C. tibetana could be accurately revealed by means of ISSR marker analysis.关键词
钩锥/ISSR标记/遗传多样性/基因分化/聚类分析Key words
Castanopsis tibetana/ISSR marker/genetic diversity/gene differentiation/cluster analysis分类
农业科技引用本文复制引用
田艳伶,李柏海,李志辉,杨模华,张斌,周鑫伟..基于ISSR标记的福建省钩锥遗传多样性分析[J].广西植物,2017,37(1):42-48,63,8.基金项目
国家林业行业公益性项目(201204405)；湖南省林业科技创新计划项目(XLK201424)[Supported by National Public Welfare Program of Forestry Industry (201204405)；Technology Innovation Plan Project in Hunan Forestry (XLK201424)]。 （201204405）
