海南医学院学报2017，Vol.23Issue(3)：296-298,302,4.DOI:10.13210/j.cnki.jhmu.20161125.004
rhPDGF 凝胶对糖尿病溃疡大鼠创面愈合及创面中炎症反应、血管新生的影响
Effect of rhPDGF gel on wound heal ing as well as inflammation and angiogenesis in the wound of rats with diabetes ulcer
摘要
Abstract
[ABSTRACT]Objective:To study the effect of recombinant human platelet-derived growth factor (rhPDGF)gel on wound healing as well as inflammation and angiogenesis in the wound of rats with diabetes ulcer.Methods:Clean male Wistar rats were selected as the research objects,made into diabetes ulcer models and then divided into model group,low dose group and high dose group who received blank gel,30 μg/g rhPDGF gel and 100 μg/g rhPDGF gel respectively for intervention.4 days, 6 days,8 days and 10 days after intervention,the area of ulcer wound was observed;6 days and 10 days after intervention, wound tissue was collected to detect the expression of inflammation and angiogenesis molecules.Results:4 days,6 days,8 days and 10 days after intervention,ulcer wound area of low dose group and high dose group were significantly less than those of model group,and ulcer wound area of high dose group were significantly less than those of low dose group;6 days and 10 days after intervention,TNF-αand IL-2 levels in ulcer wounds of low dose group and high dose group were significantly lower than those of model group while VEGF,eNOS and NO levels were significantly higher than those of model group;TNF-αand IL-2 levels in ulcer wounds of high dose group were significantly lower than those of low dose group while VEGF,eNOS and NO levels were significantly higher than those of low dose group.Conclusion:rhPDGF gel has promoting effect on the wound healing in rats with diabetes ulcer,and can also inhibit inflammation and promote angiogenesis.关键词
2 型糖尿病/溃疡创面/血小板源生长因子/炎症反应/血管新生Key words
Type 2 diabetes mellitus/Ulcer wound/Platelet-derived growth factor/Inflammation/Angiogenesis分类
医药卫生引用本文复制引用
王明才,殷小华..rhPDGF 凝胶对糖尿病溃疡大鼠创面愈合及创面中炎症反应、血管新生的影响[J].海南医学院学报,2017,23(3):296-298,302,4.基金项目
四川省卫生厅科研课题(130883) （130883）
