海南医学院学报2017，Vol.23Issue(3)：408-410,414,4.DOI:10.13210/j.cnki.jhmu.20161124.004
宫颈癌组织中 DNA 甲基转移酶表达及其与 HPV 感染、肿瘤恶性程度的关系
DNA methyltransferase (DNMTs)expression in cervical cancer tissues and its relationship with HPV infection and tumor mal ignancy
摘要
Abstract
[ABSTRACT]Objective:To study the DNA methyltransferase (DNMTs)expression in cervical cancer tissues and its rela-tionship with HPV infection and tumor malignancy.Methods:The cervical cancer tissues and normal cervical tissues surgically removed in Department of Obstetrics and Gynecology of our hospital between July 2013 and March 201 6 were collected,the fluorescent quantitative PCR method was used to determine the mRNA expression of DNMTs in the tissues,and enzyme-linked immunosorbent assay kits were used to determine the protein expression of DNMTs molecules and pro-apoptotic molecules in the tissues.Results:DNMT1,DNMT2,DNMT3a,DNMT3b and DNMT3l mRNA expression and protein expression in cervi-cal cancer tissues were significantly higher than those in normal cervical tissues while Fas,FasL,Bax and Caspase-3 protein ex-pression were significantly lower than those in normal cervical tissues;DNMT1,DNMT2,DNMT3a,DNMT3b and DNMT3l protein expression in cervical cancer tissues with high-risk HPV infection (+)were significantly higher than those in cervical cancer tissues with high-risk HPV infection (-);Fas,FasL,Bax and Caspase-3 protein expression were negatively correlated with DNMT1,DNMT2,DNMT3a,DNMT3b and DNMT3l protein expression.Conclusion:Elevated DNMTs expression in cervical cancer tissues is associated with high-risk HPV infection,and highly expressed DNMTs can inhibit the expression of pro-apoptotic molecules.关键词
宫颈癌/DNA 甲基转移酶/人乳头瘤病毒/凋亡Key words
Cervical cancer/DNA methyltransferase/Human papilloma virus/Apoptosis分类
医药卫生引用本文复制引用
李利玲,王少帅..宫颈癌组织中 DNA 甲基转移酶表达及其与 HPV 感染、肿瘤恶性程度的关系[J].海南医学院学报,2017,23(3):408-410,414,4.基金项目
国家自然科学基金资助项目(81302267) （81302267）
