海南医学院学报2017，Vol.23Issue(4)：433-436,4.DOI:10.13210/j.cnki.jhmu.20161208.004
子痫前期胎盘组织中维生素E的含量及与氧化应激损伤、细胞凋亡的相关性
Vitamin E levels in preeclampsia placenta tissue and its correlation with oxidative stress in-jury and apoptosis
摘要
Abstract
[ABSTRACT]Objective:To study the vitamin E levels in preeclampsia placenta tissue and its correlation with oxidative stress injury and apoptosis.Methods:A total of 60 pregnant women with preeclampsia who received treatment and gave birth in our hospital between July 2012 and January 201 6 were collected and divided into mild preeclampsia group (n =41)and severe preeclampsia group (n = 1 9)according to the disease severity;38 normal pregnant women who received pregnancy test and gave birth in our hospital during the same period were selected as healthy control group.The placental tissue samples of three groups of research subjects were retained,high performance liquid chromatograph-mass spectrometry was used to detect VitE levels in tissue grinding fluid,automatic biochemical analyzer was used to detect the levels of oxidative stress injury indexes, and fluorescence quantitative PCR method was used to detect the mRNA expression of apoptosis molecules.Results:VitE, SOD and CAT levels in grinding fluid of severe preeclampsia group were lower than those of mild preeclampsia group and healthy control group while ROS and AOPP levels were higher than those of mild preeclampsia group and healthy control group;Fas,caspase and Apaf-1 mRNA expression were higher than those of mild preeclampsia group and healthy control group while anti-apoptotic molecules Bcl-2,Bcl-xl,Mcl-2 and p57 k i p 2 mRNA expression were lower than those of mild pre-eclampsia group and healthy control group.Spearman correlation analysis showed that VitE level in the preeclampsia placenta tissue was directly correlated with oxidative stress injury and cell apoptosis.Conclusions:VitE deficiency is the direct factor that results in oxidative stress and cell apoptosis in patients with preeclampsia,and the VitE supplementation in time is expec-ted to become the auxiliary treatment means for patients with preeclampsia.关键词
子痫前期/维生素 E/氧化应激/细胞凋亡Key words
Preeclampsia/Vitamin E/Oxidative stress/Cell apoptosis分类
医药卫生引用本文复制引用
李军,韩玲,董力..子痫前期胎盘组织中维生素E的含量及与氧化应激损伤、细胞凋亡的相关性[J].海南医学院学报,2017,23(4):433-436,4.基金项目
国家卫生计生委医药卫生科技发展研究中心(W2016CWQT26)[Foundation Project]:It was supported by Medical and Health Science and Technology Development Research Center of National Health and Family Planning Commission (No ．W2016CWQT26)． （W2016CWQT26）
