郑州大学学报（医学版）2017，Vol.52Issue(1)：1-4,4.DOI:10.13705/j.issn.1671-6825.2017.01.001
冬凌草活性成分 KY3对食管癌 EC109细胞增殖的抑制作用
Inhibitory effect of active ingredient of Rabdosia rubescens KY 3 on pro-liferation of esophageal carcinoma EC109 cells
摘要
Abstract
Aim: To investigate the proliferation inhibition and the mechanism of active ingredients of Rabdosia rubescens KY3 on esophageal carcinoma EC109 cells.Methods:MTT assay was performed to measure the effect of 0,10, 20,30,40,60,80,100 μmol/L KY3 on EC109 cell proliferation.Flow cytometry assay was conducted to measure the effect of 0,10,20,30 μmol/L KY3 on apoptosis and cell cycle distribution of EC 109 cells, immunofluorescence was used to de-termine the effect of KY3 on microtubule structure of EC109 cells, and Hoechst 33258 staining was used to detect the mor-phological changes of the nucleus of EC 109 cells after the treatment of KY3.Results:KY3 could inhibit the proliferation of EC109 cells in vitro,and induce cell cycle arrest in G2/M phase as well as apoptosis of EC109 cells(P<0.05).After being treated by KY3, the microtubule of EC109 cells was destroyed.After the dose of KY3 increasing, α-microtubulin degradated gradually and had a typical morphological characteristic of apoptosis such as nucleus pycnosis and bright nuclei . Conclusion:The active ingredient of Rabdosia rubescens KY 3 could inhibit the proliferation of EC 109 cells in vitro, and the underlying mechanisms may be related to the destruction of microtubule , the cell cycle arrest at G 2/M phase as well as the induction of the apoptosis of EC 109 cells.关键词
EC109细胞/凋亡/周期阻滞/KY3Key words
EC109 cell/apoptosis/cycle arrest/KY3分类
医药卫生引用本文复制引用
方莉,王赛琪,陈雪梅,薛博涵,王冠涛,李玉萍,刘宏民..冬凌草活性成分 KY3对食管癌 EC109细胞增殖的抑制作用[J].郑州大学学报（医学版）,2017,52(1):1-4,4.基金项目
国家自然科学基金面上项目81430085，21372206，81172937；河南省科技厅基础与前沿项目142300410038；河南省教育厅基金资助项目17A310006；第59批国家博士后基金面上资助项目2016M592314 （）
