郑州大学学报（医学版）2017，Vol.52Issue(1)：34-36,3.DOI:10.13705/j.issn.1671-6825.2017.01.009
阿昔替尼联合塞来昔布体外抗胃癌细胞 BGC-823和 SNU-5增殖及抗血管生成作用观察
Combination efficacy of axitinib and celecoxib on proliferation of gastric cancer cells BGC-823 and SNU-5 and its antiangiogenesis effect
黄照茹 1张连峰 1周琳1
作者信息
- 1. 郑州大学第一附属医院消化内科郑州450052
- 折叠
摘要
Abstract
Aim:To explore the combination efficacy of axitinib (Ax) and celecoxib(Cel) on the proliferation of gas-tric cancer cell lines BGC-823 and SNU-5 and antiangiogenesis effect .Methods: SRB and CCK-8 methods were used to assess the effect of 10 nmol/L Cel and 1 nmol/L Ax on the proliferation of BGC-823 and SNU-5,respectively.Capillary for-mation was detected by Matrial-plug assay in vitro.Results:SRB and CCK-8 assay showed that Cel and Ax inhibited the proliferation of BGC-823 and SNU-5.The inhibitory rate of combined treatment with Cel and Ax was significantly higher than that of Cel or Ax alone ,and the combination index was lower than 1.The vascular indexes of HUVEC treated by Cel and Ax were (0.86 ±0.03) and (0.83 ±0.01), respectively, while the combined treatment group was (0.67 ±0.02) (P<0.05).Conclusion:The combination therapy of Ax and Cel reveals synergetic effect not only in the cell proliferation but also in angiogenesis inhibition .关键词
塞来昔布/阿昔替尼/胃癌/联合用药/BGC-823细胞/SNU-5细胞Key words
celecoxib/axitinib/gastric cancer/combination therapy/BGC-823 cell/SNU-5 cell分类
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黄照茹,张连峰,周琳..阿昔替尼联合塞来昔布体外抗胃癌细胞 BGC-823和 SNU-5增殖及抗血管生成作用观察[J].郑州大学学报（医学版）,2017,52(1):34-36,3.
