郑州大学学报（医学版）2017，Vol.52Issue(1)：37-41,5.DOI:10.13705/j.issn.1671-6825.2017.01.010
二甲双胍联合化疗药物对人胃癌 AG S细胞的作用
Effect of metformin combined with chemotherapeutic agents on gastric cancer cell line AGS
摘要
Abstract
Aim:To investigate the effect of metformin ( Met) combined with chemotherapeutic agents on human gas-tric cancer cell line AGS .Methods:AGS cells were treated with Met alone or in combination of cisplatin ( DDP) , doxoru-bicin(ADM) or paclitaxel(PTX).CCK-8, Transwell, and flow cytometry were used to detect cell proliferation , migration, invasion and apoptosis.Results:Met could inhibit cell proliferation in a dose-and time-dependent manner(P<0.001), and IC50 was 10 mmol/L.10 mmol/L Met,2 mg/L DDP,0.02 mg/L ADM,0.02 mg/L PTX separate application could de-crease the migration, invasion capabilities and prompt apoptosis of AGS cells (P<0.05), and Met had synergistic effected when being used with DDP, ADM or PTX in cell apoptosis(P<0.05).Conclusion: Met might be used for adjuvant chemotherapy for gastric cancer .关键词
二甲双胍/胃癌/顺铂/阿霉素/紫杉醇Key words
metformin/gastric cancer/cisplatin/doxorubicin/paclitaxel分类
医药卫生引用本文复制引用
吴诗文,张自森,刘谦,王世超,司远方,赵胜男,夏兴洲..二甲双胍联合化疗药物对人胃癌 AG S细胞的作用[J].郑州大学学报（医学版）,2017,52(1):37-41,5.基金项目
河南省医学科技攻关计划项目201403126 （）
