黑龙江农业科学Issue(12)：44-48,5.DOI:10.11942/j.issn1002-2767.2016.12.0044
好氧堆肥中amoA硝化细菌群落动态变化
Dynamic Change of amoA Nitrifying Bacteria Community During Aerobic Composting
摘要
Abstract
In order to reduce nitrogen loss during the composting ,and to optimize the composting process ,high temperature aerobic composting was conducted with cow manure and maize stalk as materials .Polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE) technique and sequence analysis method were used to analyze the dynamic change of the community composition and diversity of amoA‐nitrifying bacte‐ria during composting different periods ,which correlated with composting physio‐chemical parameters .The results showed that the nitrifying bacteria groups obviously changed after thermophilic period .Some new nitri‐fying bacteria groups were detected during cooling period .These groups belonged to Nitrosomonas sp .in the class of β‐Proteobacteria .Dominant nitrifying bacteria during composting belonged to Nitrosospira sp .in the class of β‐Proteobacteria and uncultured bacteria .The diversity index of nitrifying bacteria changed during composting process ,which decreased from mesophilic to thermophilic stage ,increased at cooling stage and then decreased at mature stage .Redundancy analysis (RDA) results showed that the ammonium nitrogen con‐tent and temperature of compost were the keys to the physio‐chemical factors significantly affected nitrifying bacteria community composition (P<0 .05) .关键词
堆肥/amoA/硝化细菌/群落结构/多样性Key words
compost/amoA/nitrifying bacteria/community structure/diversity分类
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徐莹莹,刘玉涛,王俊河,王宇先,杨慧莹,高盼,樊景胜..好氧堆肥中amoA硝化细菌群落动态变化[J].黑龙江农业科学,2016,(12):44-48,5.基金项目
公益性行业（农业）科研专项经费资助项目（201303125-21、201503116-02）；齐齐哈尔市科技局农业公关资助项目 （）
