华南农业大学学报2017，Vol.38Issue(1)：5-8,4.DOI:10.7671/j.issn.1001-411X.2017.01.002
马波沙星在罗非鱼体内的药物代谢动力学研究
Pharmacokinetics of marbofloxacin in tilapia (Oreochromis niloticus)
摘要
Abstract
Objective] To study the pharmacokinetics characteristics of marbofloxacin in tilapia ( Oreo-chromis niloticus) , and provide references for rational clinical drug usage.[Method]The tilapia were di-vided into two groups for intramuscular ( i. m. ) and oral administrations, and each fish received marbo-floxacin at a dosage of 10 mg·kg-1 body mass. Water temperature was maintained at 30 ℃. The con-centration of marbofloxacin in plasma was measured using high performance liquid chromatography ( HPLC) combined with fluorescence detection. The plasma drug concentration-time data were analyzed by the non-compartment model of WinNonlin 6. 1 software.[Result]After i. m. administration, marbo-floxacin absorption and elimination were relatively faster, and it was widely distributed throughout the body. Peak time of plasma concentration ( tmax ) was 0. 25 h, the maximum plasma concentration (ρmax ) was 4. 31 μg·mL-1 , the elimination half-life ( t1/2λz ) was 19. 21 h, the volume of distribution was 3. 94 L·kg-1 and the area under the plasma concentration-time curve ( AUC) was 70. 36 μg·mL-1·h-1 . Fol-lowing oral administration, marbofloxacin absorption and elimination were relatively slower, and it was widely distributed throughout the body. tmax was 4. 00 h, ρmax was 2. 45 μg·mL-1 , t1/2λz was 22. 67 h, the volume of distribution was 4. 27 L·kg-1 and AUC was 76. 66 μg·mL-1 ·h-1 .[Conclusion]Using 10 mg·kg-1 marbofloxacin could effectively control susceptible micro-organisms involved in most com-mon infections in tilapia.关键词
马波沙星/罗非鱼/药物代谢动力学/高效液相色谱Key words
marbofloxacin/Oreochromis niloticus/pharmacokinetics/HPLC分类
农业科技引用本文复制引用
单奇,白野,范晶晶,刘书贵,尹伊,李丽春,郑光明..马波沙星在罗非鱼体内的药物代谢动力学研究[J].华南农业大学学报,2017,38(1):5-8,4.基金项目
国家自然科学基金(31502125) （31502125）
广东省自然科学基金-博士启动基金(2015A030310086) （2015A030310086）
农业部水产品质量安全风险评估项目(GJFP201501001) （GJFP201501001）
