华南农业大学学报2017，Vol.38Issue(1)：9-14,6.DOI:10.7671/j.issn.1001-411X.2017.01.003
广州地区猫泛白细胞减少症病毒分离鉴定及全基因组遗传分析
Isolation,identification and sequence analysis of complete genome of feline panleukopenia virus in Guangzhou area
摘要
Abstract
Objective] To study the natural recombination, cross host transmission and prevalence of feline panleukopenia virus( FPLV) in Guangzhou area.[Method]Cells were isolated from stool samples of cats seemingly infected with FPLV, genome DNAs were extracted from positive samples, and complete virus genome sequences were amplified by PCR and were compared with relevant reference sequences from GenBank using genetic evolution analysis. The major amino acid sites were analyzed for differences between VP2 gene and NS1 gene. [Result]Two FPLV strains were successfully isolated and the complete genome sequences of FPLV were obtained. The genetic evolution analysis indicated that FPLV-GZ01 and FPLV-GZ02 which were isolated from Guangzhou area belonged to the same branch with the other isolated strains FPLV-XJ1 and FPLV-HRB, and implied FPLV-GZ01 and FPLV-GZ02 evolved from FPLV-XJ01. The NS1 gene phylogenetic analysis demonstrated that FPLV might have recombined with CPV-447 . Analysis of major amino acid sites indicated that FPLV was more conservative than CPV in the genetic variation of the VP2 gene. FPLV-GZ01 and FPLV-GZ02 had different degrees of mutation in the amino acid sites of the NS1 gene. [Conclusion]The feline panleukopenia viruses isolated from Guangzhou area have been still recombining and evolving.关键词
猫泛白细胞减少症病毒/细小病毒/分离鉴定/全基因组/遗传分析Key words
feline panleukopenia virus/parvovirus/isolation and identification/complete genome/genetic analysis分类
农业科技引用本文复制引用
洪马林,付橙,黄三,周沛,粟硕,李守军..广州地区猫泛白细胞减少症病毒分离鉴定及全基因组遗传分析[J].华南农业大学学报,2017,38(1):9-14,6.基金项目
国家重点研发计划(2016YFD0501004) （2016YFD0501004）
公益性行业(农业)科研专项(201303042) （农业）
