华南农业大学学报2017，Vol.38Issue(1)：50-57,8.DOI:10.7671/j.issn.1001-411X.2017.01.009
水稻根际土壤溶磷菌的分离、鉴定及对水稻的促生作用
Isolation and identification of phosphate solubilizing bacteria from rice rhizosphere soil, and promoting effect on rice plant growth
摘要
Abstract
Objective] To determine the ability of phosphate dissolving of phosphate solubilizing bacteria ( PSB) in rice rhizosphere, and study the effect of PSB on soluble phosphorus contents in rice plants and rhizosphere soil. [Method] Two bacteria strains NDW1 and NDW3 with strong ability of dissolving Ca3 ( PO4 ) 2 were isolated from rice rhizosphere soil based on the halo zone formation on media due to phosphate solubilization, and identified by 16S rDNA method. Phosphorus dissolving conditions of two strains were optimized by orthogonal test with NBRIP as the basic medium. The production of indole-3-acetic acid( IAA) were determined. The effects of NDW1 and NDW3 on promotion of rice growth, the soil available phosphorus contents and plant total phosphorus contents were studied. [Result]NDW1 and NDW3 were identified as Enterobacter sp. and Serratia sp. . The best combination of carbon, nitrogen source and pH for phosphate solubilition by NDW1 and NDW3 were both the combination of glucose, peptone and pH 6 . The highest phosphorus contents dissolved by NDW1 and NDW3 were 294. 95 and 312. 93 μg · mL-1 after 24 h incubation, respectively. Both PSB could produce IAA. NDW3 significantly increased the shoot height, root length, maximum leaf length and above-ground dry mass of rice plant in both soil culture and sand culture. NDW3 also significantly increased the contents of soil available phosphorus and rice total phosphorus under both culture conditions.[Conclusion]Enterobacter sp. NDW1 and Serratia sp. NDW3 isolated from rice rhizosphere soil have strong ability of phosphate dissolving. The growth promoting effect of NDW3 for rice plant is better than that of NDW1.关键词
土壤溶磷菌/IAA/促生作用/磷含量/水稻/根际Key words
soil phosphate solubilizing bacteria/IAA/growth promoting effect/phosphorus content/rice/rhizosphere分类
农业科技引用本文复制引用
武志海,孙合美,杨美英,卢冬雪,岳胜天,付丽..水稻根际土壤溶磷菌的分离、鉴定及对水稻的促生作用[J].华南农业大学学报,2017,38(1):50-57,8.基金项目
国家自然科学基金(31201687) （31201687）
