华南农业大学学报2017，Vol.38Issue(1)：63-68,6.DOI:10.7671/j.issn.1001-411X.2017.01.011
球孢白僵菌与玫烟色棒束孢制剂对柑橘木虱的防治
The biocontrol effects of Beauveria bassiana and Isaria fumosorosea on Asian citrus psyllid
摘要
Abstract
Objective] To study pathogenicity and biological control effects of two entomopathogenic fungi Beauveria bassiana and Isaria fumosorosea to the nymphs and adults of Asian citrus psyllid ( ACP ) Diaphorina citri. [Method] The spore suspensions of B. bassiana and I. fumosorosea were diluted to 1 × 104 , 1 × 105 , 1 × 106 , 1 × 107 and 1 × 108 mL-1 , and then were sprayed on ACP younger instar, older instar nymphs and adults indoor to study the lethal effects and under semi-field conditions to study the control effects against ACP adult population. [Result] B. bassiana and I. fumosorosea had higher pathogenicity to younger nymphs ( 2 -3 instar ) than to older nymphs ( 4 -5 instar ) . After seven days infection, the LC50 of B. bassiana and I. fumosorosea to ACP older nymphs were 3. 6 × 104 and 5. 2 × 104 mL-1 , to ACP younger nymphs were 3. 5 × 104 and 4. 2 × 104 mL-1 , and to ACP adults were 1. 4 × 105 and 1. 6 × 105 mL-1 respectively. Under semi-field conditions, the LC50 of B. bassiana and I. fumosorosea to ACP adults increased to 3. 7 × 105 and 1. 2 × 106 mL-1 . The lethal effects of two fungi at the same spore concentration against ACP in semi-field were lower than those indoor. Meanwhile, the lethal time of both fungi to ACP adults was correlated with spore concentration. The LT50 decreased with the increase of spore concentration, ranged between 5. 2-4. 4 days when the spore concentration of B. bassiana varied from 1 × 105 to 1 × 108 mL-1 , and ranged between 5. 3 -4. 9 days when the spore concentration of I. fumosorosea varied from 1 × 106 to 1 × 108 mL-1 . [Conclusion] Both B. bassiana and I. fumosorosea have good biocontrol effects against ACP. The fatality rates of ACP are positively correlated with the concentrations of fungal spores.关键词
柑橘木虱/球孢白僵菌/玫烟色棒束孢/致病力/生物防治Key words
Asian citrus psyllid/Beauveria bassiana/Isaria fumosorosea/pathogenicity/biological control分类
农业科技引用本文复制引用
代晓彦,李翌菡,沈祖乐,许炜明,吴建辉,任顺祥,邱宝利..球孢白僵菌与玫烟色棒束孢制剂对柑橘木虱的防治[J].华南农业大学学报,2017,38(1):63-68,6.基金项目
广东省科技计划(2015A020209140) （2015A020209140）
广州市科技计划(201509010023) （201509010023）
广州市产学研协同创新重大专项对外合作项目(201604030029) （201604030029）
广东省普通高校省级重大科研项目(2014-16) （2014-16）
