华南农业大学学报2017，Vol.38Issue(2)：1-5,5.DOI:10.7671/j.issn.1001-411X.2017.02.001
HCl 和 DMSO 阻断滞育条件下桑蚕卵壳电镜观察
SEM observation on silkworm eggshell under HCl or dimethyl sulfoxide (DMSO) treatment for preventing diapause
摘要
Abstract
Objective] To investigate the diapause prevention mechanism of silkworm ( Bombyx mori ) , and compare a series of changes of eggshell surface after diapause prevention by HCl or DMSO treatment .[Method]The eggs were treated with HCl or DMSO at 20 and 12 h after oviposition respectively .Scan-ning electron microscope ( SEM ) was used to observe the changes of eggshell surface and aeropyle at different time points after treatment .[Result]Both HCl and DMSO treatments successfully prevented the occurrence of egg diapause , and the average hatching rates were 93.37% and 87.81% respectively . SEM observation showed clear etching effect and “lipid” shed on eggshell surface , and the aeropyle diameter increased gradually under both treatments .The above observations were more obvious for the HCl treatment compared with the DMSO treatment .[Conclusion]Eggshell surface changes may be related to the diapause prevention of silkworm .关键词
桑蚕/阻断滞育/HCl/DMSO/扫描电镜/卵壳Key words
silkworm/diapause prevention/HCl/DMSO/scanning electron microscopy/eggshell分类
农业科技引用本文复制引用
龚竞,田莎,侯勇,赵欢欢,朱勇..HCl 和 DMSO 阻断滞育条件下桑蚕卵壳电镜观察[J].华南农业大学学报,2017,38(2):1-5,5.基金项目
973计划（2012CB114600）；国家自然科学基金（31201853）；重庆市基础与前沿研究计划 （）
