华南农业大学学报2017，Vol.38Issue(2)：12-17,6.DOI:10.7671/j.issn.1001-411X.2017.02.003
新西兰白兔中Myh6基因的表达及与肌纤维性状的相关性
Myh6 gene expression and its correlation with muscle fiber traits of New Zealand white rabbits
摘要
Abstract
Objective] To analyze Myh6 gene expression and its correlation with the muscle fiber traits of New Zealand white rabbits , and explore the role of Myh6 gene during the development of muscle fibers.[Method]Density, area, long and short diameters for back and leg muscle fibers were measured for 2-, 4-, 6-, 8-, 10-and 12-week-old New Zealand white rabbits using the frozen section technique .Myh6 gene expressions in back and leg muscles of rabbits at different ages were estimated using qRT-PCR.The correlations between Myh6 gene expression and muscle traits were analyzed .[Result] The density of various muscle fibers gradually decreased , but the area , long and short diameters of muscle fibers increased from two to eight weeks , except for the short diameters of white muscle fibers increased from two to six weeks .The expression level of Myh6 gene was the highest in 4-week-old rabbit .The expression level of Myh6 gene was significantly ( P<0.01 ) negatively correlated with the red muscle fiber area of male rabbit back muscles , and significantly ( P<0.01 ) positively correlated with the red muscle fiber density of male rabbit leg muscles .[Conclusion] Myh6 gene expression is closely related to type and growth of muscle fibers .关键词
兔/肌纤维/冰冻切片/Myh6/相关性分析Key words
rabbit/muscle fiber/frozen section/Myh6/correlation analysis分类
农业科技引用本文复制引用
胡帅帅,王文洲,闫晓荣,赵博昊,朱杰,郝晔,陈阳,吴信生..新西兰白兔中Myh6基因的表达及与肌纤维性状的相关性[J].华南农业大学学报,2017,38(2):12-17,6.基金项目
现代农业产业技术体系建设专项（CARS-44-1）；江苏高校优势学科建设工程资助项目 （）
