华南农业大学学报2017，Vol.38Issue(2)：27-31,5.DOI:10.7671/j.issn.1001-411X.2017.02.006
广州市售肉类食品源大肠埃希菌耐药性分析
Antimicrobial activity of Escherichia coli from retail meat in Guangzhou
摘要
Abstract
[Objective] To understand the contamination status and antimicrobial activity of Escherichia coli in retail meat sold in Guangzhou .[Method]We collected 310 meat samples including 253 retail pork and 57 chicken meat from markets and supermarkets in Guangzhou from July to August , 2011.E.coli strains were isolated and identified using the selective media .The susceptibilities of E.coli strains to 19 common antibiotics represented by the minimum inhibitory concentrations ( MIC ) were tested using the agar dilution method .[Result]Totally 213 E.coli strains were isolated from 310 meat samples with the detection frequency of 68.7%, including 117 pork isolates and 36 chicken meat isolates .More than 75.0%isolates were resistant to sulfamethoxazole/trimethoprim , streptomycin and tetracycline , 10.0%to 60.0%isolates were resistant to ampicillin , nalidixic acid , chloramphenicol , neomycin , florfenicol , fosfomycin, ciprofloxacin, apramycin, enrofloxacin, gentamicin and cefazolin, and the isolates were sen-sitive to cefoxitin , ceftriaxone , ceftazidime , colistin and amikacin with below 5.0% being resistant strains.Isolates from chicken meat showed significantly higher resistance to cefazolin , cefoxitin, ceftriax-one, ceftazidime and fosmycin compared to pork isolates ( P <0.01 ) .There were 82.2% multi-drug resistant strains of 213 E.coli strains.[Conclusion]Retail meat sold in Guangzhou were severely contami-nated by multi-drug resistant E.coli, and chicken meat isolates showed higher resistance compared to pork isolates .关键词
大肠埃希菌/肉类食品源/琼脂稀释法/耐药性Key words
Escherichia coli/retail meat origin/agar dilution method/antimicrobial activity分类
农业科技引用本文复制引用
曾莉,黄仕凤,刘思玲,王晶,刘健华..广州市售肉类食品源大肠埃希菌耐药性分析[J].华南农业大学学报,2017,38(2):27-31,5.基金项目
973计划 （）
