华南农业大学学报2017，Vol.38Issue(2)：69-74,6.DOI:10.7671/j.issn.1001-411X.2017.02.013
Serratia sp．NDW3 GADH 小亚基基因ga2 dh 的克隆及表达分析
Cloning and expression analysis of GADH small subunit gene ga2dh from Serratia sp.NDW3
摘要
Abstract
[Objective] To clone and identify GADH small subunit gene ga2dh.[Method] Changes in soluble phosphorus content , GADH activity and ga2dh gene expression level during the phosphate solubi-lizing process of Serratia sp.NDW3 from rice rhizosphere were studied .The ga2dh gene was cloned and expressed in Escherichia coli BL21, and bioinformatic analysis was performed .[Result] The relative expression of ga2dh gene in the process of phosphate solubilizing by Serratia sp.NDW3 reached maxi-mum at 12 h, GADH activity reached maximum at 24 h, and the soluble phosphorus content stabilized after 36 h.The ga2dh gene sequence of 781 bp was obtained from Serratia sp.NDW3 by cloning.The similarity between ga2dh gene and Serratia sp.SCBI sequences was 99.62% based on bioinformatic analysis.The protein encoded by ga2dh belonged to the superfamily of gluconate dehydrogenase subunit 3 and was composed of three α-helices.The amino acid sequence was consisted of intracellular , extracellu-lar and transmembrane regions.The expression of ga2dh gene in E.coli BL21 significantly increased GADH activity.[Conclusion] The main mechanism of phosphate solubilizing by Serratia sp.NDW3 is through the direct oxidation pathway .The small subunit encoded by ga2dh gene not only plays an impor-tant role in GADH activation , but also is part of the transmembrane structure of GADH .关键词
水稻/溶磷菌/GADH/ga2dh/基因表达/酶活性Key words
rice/phosphate solubilizing bacteria/GADH/ga2dh/gene expression/enzyme activity分类
农业科技引用本文复制引用
杨美英,卢冬雪,孙合美,武志海,岳胜天,付丽..Serratia sp．NDW3 GADH 小亚基基因ga2 dh 的克隆及表达分析[J].华南农业大学学报,2017,38(2):69-74,6.基金项目
国家自然科学基金 （）
