河南农业科学2017，Vol.46Issue(2)：6-11,6.DOI:10.15933/j.cnki.1004-3268.2017.02.002
冬小麦膨胀素基因TaEXPB8、TaEXPB10的克隆及低温表达分析
Cloning of TaEXPB8 and TaEXPB10 in Winter Wheat and Their Expression Analysis under Low Temperature Condition
摘要
Abstract
The cold-resistant winter wheat variety Dongnongdongmai No. 1 was used as experimental ma-terial. The expansin genes TaEXPB8 and TaEXPB10 were cloned from its roots,and their expression pro-files were analyzed under low temperature conditions,so as to lay a foundation for study of the relationship between expansin genes expression and the plant cold resistance. The results showed that the expansin genes TaEXPB8 and TaEXPB10 were cloned from cold-resistant winter wheat variety Dongnongdongmai No. 1,which coded 273 and 271 amino acids,respectively. In addition,they shared 99. 2% and 99. 6%homology separately with the published sequence in GenBank. Besides,4 ℃,-10 ℃ and -20 ℃ could induce the expression of TaEXPB8 and TaEXPB10 in roots of Dongnongdongmai No. 1. Especially for TaEXPB10,it had higher inducing level than TaEXPB8. However,these two genes were not induced in Ji-mai No. 22 ( cold susceptible variety) on a whole,even were suppressed. Taken together,we found that under 4 ℃,-10 ℃ and -20 ℃,the expression level of TaEXPB8 and TaEXPB10 in Dongnongdongmai No. 1 (cold resistant variety) were all significantly higher than that in Jimai No. 22(cold susceptible vari-ety) . Thus,it had been postulated that the expansin genes TaEXPB8 and TaEXPB10 had great relevance with low temperature stress tolerance ability of the roots of Dongnongdongmai No. 1. It also provided fur-ther understanding on the cold resistance mechanism in winter wheat root system.关键词
冬小麦/膨胀素/低温处理/表达分析Key words
winter wheat/expansin/low temperature treatment/expression analysis分类
农业科技引用本文复制引用
王明晶,王晓磊,徐永清,李凤兰,王旭,彭丽娜,李飞,董佳敏,张俊峰..冬小麦膨胀素基因TaEXPB8、TaEXPB10的克隆及低温表达分析[J].河南农业科学,2017,46(2):6-11,6.基金项目
国家基础科学人才培养基金(J1210069) （J1210069）
