河南农业科学2017，Vol.46Issue(2)：120-123,4.DOI:10.15933/j.cnki.1004-3268.2017.02.025
牛BBOX1基因3'UTR变异体的克隆及其多态性分析
Cloning of 3'UTR Isoforms and Polymorphisms in Bovine BBOX1 Gene
摘要
Abstract
The objective of this study was to identify isoforms of 3'UTR and polymorphisms in 3'UTR regions of bovine BBOX1 gene. Three different alternative polyadenylation(APA) isoforms of 3'UTR in the BBOX1 gene were identified by 3′RACE. Their lengths were 229,664,678 bp, respectively. Ten polymorphisms loci were identified in 3'UTR of BBOX1 gene by single strand conformation polymorphism (SSCP) and sequencing. There were seven SNPs,including c. 12T>C,c. 100A>G,c. 241T>C,c. 480T>C, c. 557T>C, c. 606T >C, and c. 650T >C. Three were deletion/insertion mutations, including c. 2829insC,c. 434435delGT,and c. 512513insTGC. SNP c. 650T>C was located in polyadenylation signals sequence PAS3. Moreover,the mutation changed AAUAAA into AACAAA,resulting in presence of 678 bp isform of 3'UTR in bovine BBOX1 gene.关键词
牛/BBOX1基因/选择性多聚腺苷酸化/多态性/3'UTRKey words
cattle/BBOX1 gene/APA/polymorphisms/3'UTR分类
农业科技引用本文复制引用
宋雨霏,郭彦,辛友志,崔建伟,周国利..牛BBOX1基因3'UTR变异体的克隆及其多态性分析[J].河南农业科学,2017,46(2):120-123,4.基金项目
国家自然科学基金项目(31571274) （31571274）
山东省自然科学基金项目(ZR2015CM025) （ZR2015CM025）
聊城大学博士启动基金项目(31805) （31805）
