河南农业科学2017，Vol.46Issue(2)：131-135,5.DOI:10.15933/j.cnki.1004-3268.2017.02.028
枯草芽孢杆菌J-4菌株产抑菌物质发酵条件优化
Optimization of Fermentation Conditions for Producing Antibacterial Substance from Bacillus subtilis Strain J-4
摘要
Abstract
In order to improve the content of antibacterial substance in the fermentation broth of Bacillus subtilis strain J-4,the combined method of single factor test and orthogonal test was used to optimize the factors,such as carbon source,nitrogen source,inorganic salts,fermentation time,media amount,fermenta-tion temperature and inoculation amount. The medium composition and fermentation conditions after opti-mization were as follows:glucose 1%, soybean cake powder 1%, MgSO40. 15%, Na2 HPO40. 10%, NaH2PO40. 10%,fermentation time 36 h,media amount 100 mL(250 mL),fermentation temperature 37 ℃,inoculation amount 5%. The inhibition area after optimization increased by 50. 25% compared with the inhibition area when using the basal fermentation medium.关键词
枯草芽孢杆菌/抑菌物质/优化/正交试验Key words
Bacillus subtilis/antibacterial substance/optimization/orthogonal test分类
农业科技引用本文复制引用
翟玉洁,邓祖丽颖,姜军坡,王世英..枯草芽孢杆菌J-4菌株产抑菌物质发酵条件优化[J].河南农业科学,2017,46(2):131-135,5.基金项目
石家庄市科学技术研究与发展计划项目(151500062A) （151500062A）
