河南农业科学2017，Vol.46Issue(2)：148-152,5.DOI:10.15933/j.cnki.1004-3268.2017.02.031
龙利叶多酚提取条件优化及其抗氧化活性研究
Optimization of Extraction Conditions and Antioxidant Activities of Polyphenols from Sauropus rostratus Miq.
摘要
Abstract
Based on single factor test,extraction conditions of polyphenols from Sauropus rostratus Miq. were optimized by orthogonal test,and antioxidant activities of the polyphenols were investigated to provide scientific basis for the development and application of the polyphenols. Ethanol was used for polyphenols extraction. The factors such as ethanol concentration, solid-liquid ratio, extraction temperature, time and extraction times were studied. The optimum extraction conditions were obtained by orthogonal experiment and determined as follows:60% ethanol,solid-liquid ratio of 1:30,60 ℃,30 min and the extraction rate of polyphenols from Sauropus rostratus Miq. was 4. 38 mg/g. The 50% inhibition concentrations(IC50) of polyphenols from Sauropus rostratus Miq. and ascorbic acid were 1. 91 g/mL and 6. 75 g/mL respectively for scavenging DPPH ·. Reducing power was determined, and concentrations of polyphenols extracted and ascorbic acid were 9 . 94 g/mL and 32 . 06 g/mL respectively with absorbance of 0 . 5 . Results showed that free radical scavenging activities on DPPH · and reducing power of polyphenols from Sauropus ros-tratus Miq. were better than those of ascorbic acid.关键词
龙利叶/多酚/提取/正交试验/抗氧化活性Key words
Sauropus rostratus Miq./polyphenols/extraction/orthogonal test/antioxidant activities分类
农业科技引用本文复制引用
张素斌,张欣,李敏..龙利叶多酚提取条件优化及其抗氧化活性研究[J].河南农业科学,2017,46(2):148-152,5.基金项目
广东省高等学校优秀青年教师培养计划项目(Yq2013164) （Yq2013164）
肇庆市科技创新计划项目(2014G22) （2014G22）
