湖南中医药大学学报2017，Vol.37Issue(1)：65-69,5.DOI:10.3969/j.issn.1674-070X.2017.01.017
电针对佐剂性关节炎大鼠关节滑膜细胞内TAK1表达的影响
Effects of Electroacupuncture on TAK1 Expression in Articular Synovial Cell of Rats with Adjuvant-Induced Arthritis
摘要
Abstract
〔Abstract〕 Objective To observe the effect of electroacupuncture on Zusanli, Guanyuan acupoints on expression of Transforming growth factor-βactivation of Protein kinase (TAK1) in articular synovial cell of rats with adjuvant-induced arthri-tis (AIA), and explore the possible intervention mechanisms of electroacupuncture. Methods 40 SD rats were randomly divided into blank group, model group, Methotrexate (MTX) group, the electroacupuncture group, 10 rats in each group. The A-IA model was made according to the Fueund's complete adjuvant (FCA) method. The electroapuncture group rats were given electroacupuncture at Zusanli and Guanyuan point for 20 minutes everyday, 3 weeks in all. The MTX group rats were treated with MTX suspension at the dosage of 0.35 mg/kg twice a week since the injection day, 3 weeks in total. The weight, paw volume and arthritis scores of rats were measured every 3 days. The TNF-α was detected by using radioimmunoassay. The expressions of TAK1, NF-κB in ankle synovial cell of rats were detected by Western Blot. The TNF-α, TAK1, NF-κB of each group were compared after intervention. Results Compared with the normal group, the body weight growth in model group was slow, the feet swelling was significantly severe, arthritis scores increased significantly (P<0.01) and the TNF-α significantly increased, TAK1 in synovial cell significantly increased, NF-κB expression significantly increased faster(P<0.01). Compared with the model group, the body weight in electropuncture group increased faster, the feet swelling was slighter, and arthritis scores got lower (P<0.05), the TNF-α ,TAK1 and NF-κB expression in synovial cells were lower (P<0.05). Conclusion Electroacupuncture on Zusanli, Guanyuan of AIA rats is effective, its intervention mechanism may be related to inhibiting the expression of TAK1 in synovial cells, then beneficial regulating on NF-κB the inflammatory signaling path-ways.关键词
转化生长因子β激活激酶1/NF-κB/电针/佐剂性关节炎/足三里/关元穴Key words
TAK1/NF-κB/electroacupuncture/adjuvant induce arthritis/Zusanli acupoint/Guanyuan acupoint分类
医药卫生引用本文复制引用
刘梨,祁芳,李艳玲,艾坤,蔡雄,李鑫,张泓..电针对佐剂性关节炎大鼠关节滑膜细胞内TAK1表达的影响[J].湖南中医药大学学报,2017,37(1):65-69,5.基金项目
国家自然科学青年基金(81303048)；湖南省科技厅科技计划一般项目(2014SK3046)；湖南省教育厅科学研究优秀青年项目(15B176)；湖南省教育厅科学研究项目(14C0854)。 （81303048）
