海洋科学2016，Vol.40Issue(11)：1-6,6.DOI:10.11759/hykx20150206001
海带转录组SSR序列特征及其相关基因功能分析
Characterization of SSR in Saccharina japonica transcriptome and functional analysis of SSR-containing unigenes
摘要
Abstract
In this study, we identified a total of 9 237 SSR (simple sequence repeat) loci in 70 497 unigenes from the transcriptome data of Saccharina japonica and conducted a function analysis of unigenes containing SSR. SSRs were abundant in the transcriptome of S. japonica, and the most commonly repeating motifs were mononucleotides and trinucleotides (40.9% and 39.4% of the total SSRs, respectively), followed by tetranucleotides, dinucleotides, pentanucleotides, and hexanucleotides (9.8%, 6.1%, 3.1%, and 0.7% of the total SSRs, respectively). There were 147 repeated motif types ranging from 5 to 70 repetitions. Our analysis of the functional annotation revealed that nearly 50%of the unigenes containing SSRs contain annotation information and that most of them are homologous to known proteins. Our clusters of orthologous group (COG) classification and gene ontology (GO) assignment results indicate that genes containing SSR are involved in various biological functions, of which carbohydrate metabolism and cell components are the top two. These SSRs provide new resources for further developing functional SSR markers, which can play important roles in molecular marker-assisted selection while breeding S. japonica.关键词
微卫星/转录组/简单序列重复(SSR)/海带(Saccharina japonica)Key words
microsatellite/transcriptome/simple sequence repeat (SSR)/Saccharina japonica分类
农业科技引用本文复制引用
李秋莹,张杰,姚建亭,王秀良,段德麟..海带转录组SSR序列特征及其相关基因功能分析[J].海洋科学,2016,40(11):1-6,6.基金项目
国家自然科学基金项目(31272660) （31272660）
国家科技支撑计划项目(2013BAB01B01) （2013BAB01B01）
国家海洋公益性行业科研专项(201405040) （201405040）
