海洋科学2016，Vol.40Issue(11)：7-16,10.DOI:10.11759/hykx20160125001
热稳定-卡拉胶酶产生菌Bacillus sp.Car19的发酵条件优化
Optimization of fermentation conditions for thermostableκ-carrageenase production of strain Bacillus sp. Car19
摘要
Abstract
In this study, we identified a high-yield κ-carrageenase bacteria strain by its 16S rDNA sequence and optimized the fermentation conditions using a response surface methodology. The results show that the hot-spring bacterium was 99%identical to Bacillus sp. and therefore we assigned it to the Bacillus genus and named it Bacillus sp. Car19 (GenBank accession number: KT865196). The optimized fermentation conditions of Bacillus sp. Car19 involve nine factors that affect Bacillus sp. Car19 carrageenase production, and the three main factors are culture temperature, Cu2+concentration, and NaCl concentration. Combined with subordinate factors, the final optimized fermentation conditions for maximal activity, as determined by regression analysis are as follows:a culture temper-ature of 52.31℃, a Cu2+ concentration of 6.93 mmol/L, an NaCl concentration of 37.03 g/L, medium pH = 6, 1%inoculation quantity, 36 h incubation time, a galactose concentration of 0.3 g/L, an ammonium nitrate concentration of 7 g/L, and a carrageenan concentration of 0.5 g/L. The supernatant carrageenase activity after optimized fermen-tation can reach 15.82 U/mL, which is 1.5 times that without optimization.关键词
热泉菌/卡拉胶酶/优化/响应面Key words
Hot spring bacterium/Carrageenase/Optimization/Response surface分类
生物科学引用本文复制引用
谢买胜,李江,林学政,何培青..热稳定-卡拉胶酶产生菌Bacillus sp.Car19的发酵条件优化[J].海洋科学,2016,40(11):7-16,10.基金项目
海洋公益性行业科研专项项目(201505026) （201505026）
青岛市应用基础研究计划项目(14-2-4-14-jch) （14-2-4-14-jch）
国家海洋局海洋生物活性物质与现代分析技术重点实验室开放基金(MBSMAT-2015-06) （MBSMAT-2015-06）
