海洋科学2016，Vol.40Issue(11)：28-33,6.DOI:10.11759//hykx20151109002
常压室温等离子体技术诱变选育鲅鱼抗氧化肽发酵菌株
A mutant strain of Spanish mackerel antioxidant peptide fer-mentation obtained by atmospheric and room temperature plasma
摘要
Abstract
The processed waste of Spanish mackerel is rich in proteins. Antioxidant active peptide preparation via microorganism fermentation can efficiently utilize these proteins and reduce the environmental pollution caused by S. mackerel processed waste. One strain of Bacillus Hy-2 was obtained using the processed waste of aquatic prod-ucts as the substrate;this resulted in the production of protein hydrolysates with high antioxidant activity. The an-tioxidant activity of protein hydrolysates was further improved via mutation of the original strain of protease pro-ducing Bacillus Hy-2 using atmospheric and room temperature plasmas. A strain of Bacillus Hy-23 with a strong hereditary stability was screened from these mutants;this strain continued to allow a stable enzyme production even after eight generations. Using waste proteins obtained from S. mackerel production as the substrate and an inocu-lating mutant strain Hy-23, the fermentation process allowed the production of protein hydrolysates with a 12.4%increase in antioxidant activity.关键词
常压室温等离子体/蛋白酶/抗氧化活性/遗传稳定性Key words
Atmospheric and room temperature plasmas(ARTP)/protease/antioxidant activity/hereditary stability分类
生物科学引用本文复制引用
艾冰花,艾丽花,李秉钧,冯俊荣,韩龚文,于本淑..常压室温等离子体技术诱变选育鲅鱼抗氧化肽发酵菌株[J].海洋科学,2016,40(11):28-33,6.基金项目
山东省科技发展计划项目(2012GHY11515) （2012GHY11515）
