林业科学2016，Vol.52Issue(12)：150-155,6.DOI:10.11707/j.1001-7488.20161218
偏肿革裥菌产漆酶活性的诱导
Induction on Laccase Activity Produced by Lenzites gibbosa
摘要
Abstract
Objective]In order to enhance laccase activity of Lenzites gibbosa significantly,induction effects of different inducers and their optimal levels were studied.[Method]The effects of 12 kinds of inducers on laccase activity was determined under liquid culture through single factor tests. Several single factor screening tests consisting of optimal inducer’s concentration,2 inducers combination,Cu2 + concentration,inoculum volume,and static or shaking culture were conducted to gain further optimal conditions.[Result]The effects of biological inducer wheat bran and chemical inducer guaiacol on laccase production were better,and a high laccase activity was obtained when added 0. 2 mmol·L -1 Cu2 +,the optimum levels of them were 1. 5% and 1. 00 mmol·L -1 ,respectively; when one of them alone added,L. gibbosa laccase activity would reached 1 873. 16 U·L -1 and 1 975. 72 U·L -1 ,which were 13. 0 and 13. 7 times than the laccase activity of the initial medium,respectively. When 1. 5% wheat bran and 1. 00 mmol·L -1 guaiacol were added simultaneously,the laccase activity was higher than that of each one of them alone added,and the peak value of laccase activity would reached 3 594. 46 U·L -1 on 11 day under 150 r·min -1 shaking condition,which was 24. 9 times than that of the initial medium. [Conclusion]Two inducers combination could enhance laccase activity substantially,the increase in laccase production induced by them was more obvious than that of culture condition optimization.关键词
白腐菌/偏肿革裥菌/诱导剂/漆酶/酶活性Key words
white-rot fungi/Lenzites gibbosa/inducer/laccase/enzyme activity分类
生物科学引用本文复制引用
石亚攀,池玉杰,于存..偏肿革裥菌产漆酶活性的诱导[J].林业科学,2016,52(12):150-155,6.基金项目
东北林业大学博士研究生自主创新基金项目(2572015AA32) （2572015AA32）
黑龙江省自然科学基金项目(C2016006)。 （C2016006）
