广东石油化工学院学报2016，Vol.26Issue(6)：15-19,5.
固相萃取－高效液相色谱测定果酒中的有机酸磁
Determination of Organic Acids in Fruit Wines by Solid-phase Extraction and HPLC
摘要
Abstract
A rapid method for determination of organic acids in fruit wines is established .The optimal conditions are as follows :Thermo C18 reversed-phase column (4 .6 × 250 mm ,5μm ) is used;0 .01 mol/L NH4 H2 PO4 of pH 2 .55 is used as mobile phase ;flow rate is 1 .2 mL/min;the column temperature is set at 45 ℃ ;and measurement wavelength is 210 nm .10 organic acids are separated well in 9 min-utes .The peak areas and concentrations show good correlations ( R>0 .998 24 ) .The method has good reproducibility and high accuracy when RSD is in the range of 0 .25% ~3 .84% ( n=5) and the recovery is 77 .27% ~104 .56% .Organic acids in plum wine ,cherry rum ,blueberry wine ,loquat wine ,lychee wine ,red wine and mulberry wine can be determined by this method ,and the satisfactory results can be obtained .关键词
固相萃取/高效液相色谱/有机酸/果酒Key words
Solid-phase extraction/HPLC/Organic acid/Fruit wine分类
轻工纺织引用本文复制引用
陆燕,曾霞,曹建平..固相萃取－高效液相色谱测定果酒中的有机酸磁[J].广东石油化工学院学报,2016,26(6):15-19,5.基金项目
=广东石油化工学院自然科学研究项目（513036）；茂名市科技计划项目 （）
