南京工业大学学报（自然科学版）2017，Vol.39Issue(1)：64-68,5.DOI:10.3969/j.issn.1671-7627.2017.01.011
两种絮凝剂纯化猴头菇总多糖工艺的对比
Comparison of purification of polysaccharides from Hericiumerinaceus with two flocculants
摘要
Abstract
The purification of the polysaccharide solution obtained from Hericiumerinaceus by chitosan and ZTC1+1Ⅱ flocculants were studied with the deprotobeinizing rate and the polysaccharides retention rate as the evaluation indicators. The effects of factors such as dosage of flocculant, flocculation time, and flocculation temperature on the flocculation were investigated by single⁃factor experiments, and the optimum parameters were obtained.Results showed that the flocculation effect of chitosan was better than ZTC1+1Ⅱ flocculants, and the optimum flocculation parameters were as follows:the temperature of 50℃,the amount of chitosan of 0�8 mL/g ( flocculant volume/hericium amount ) and flocculation time of 2�0 h. Under the optimum conditions, the deproteinizing rate was 38�52% and the polysaccharides retention rate was 94�65%. 1,1⁃diphenyl⁃2⁃picrylhydrazyl⁃radical ( DPPH·)⁃scavenging assay was also carried out to evaluate the bioactivity of polysaccharide solution of hericium purified by chitosan and ZTC1+1Ⅱ flocculant with a better bioactivity by chitosan.关键词
猴头菇/多糖/壳聚糖/ZTC型絮凝剂Key words
Hericiumerinaceus/polysaccharide/chitosan/ZTC flocculant分类
医药卫生引用本文复制引用
豆欣欣,韩伟..两种絮凝剂纯化猴头菇总多糖工艺的对比[J].南京工业大学学报（自然科学版）,2017,39(1):64-68,5.基金项目
上海市科技兴农重点攻关项目［沪农科攻字（2012）第2－9号］ （）
