山东中医药大学学报2017，Vol.41Issue(1)：81-85,5.
清肠化湿方对TNBS大鼠结肠组织PPAR-γ/p38 MAPK的影响
Effects of Qingchang Huashi Decoction on Expression of PPAR-γ and p38 MAPK in Colonic Mucosa of Rats with Ulcerative Colitis
摘要
Abstract
Objective:To investigate the efficacy of Qingchang Huashi decoction (QHD ) on ulcerative colitis induced by 2 , 4 , 6- trinitrobenzen sulfonic acid ( TNBS ) in Wistar rats and determine the expression of PPAR-γ and p38 MAPK in colonic mucosa of rats in further to study the function and mechanism of QHD in rats with ulcerative colitis. Methods:Eighty male Wistar rats were used to establish UC models and di-vided randomly into eight groups:the normal group,model group,QHD-low group,QHD-medium group, QHD-high group,SASP group,SASP+BADGE group and the QHD-medium+BADGE group. Each group had ten rats. TNBS was used in seven groups except for the normal group to establish ulcerative colitis model in rats. SASP and BADGE which were the inhibitors of PPAR-γ were used. After 7 days,the colonic pathological morphology was observed. Western blot and Real-time PCR(RT-PCR) were performed to detect the protein levels and mRNA levels of PPAR-γ and p38 MAPK. The expressions of MUC2 and TFF3 in colonic mucosa were also measured by RT-PCR. Results:Compared with the model group,QHD could effec-tively improve the life quality,reduce colon tissue inflammatory cells infiltration,enhance colonic mucosal tissue repair and promote the recovery of UC rats. QHD could increase PPAR-γ expression and inhibit p38 MAPK activity. In addition,QHD was able to increase significantly the expressions of MUC2 and TFF3 in colonic mucosa. Conclusion:QHD had ob-vious protective effect on UC. One of the mecha-nisms might be that QHD could active PPAR-γsignaling and inhibit p38MAPK activation,increase the expression of MUC2 and TFF3 in colonic tissue to participate in restoration of intestinal mucosa ,and inhibit the release of inflammatory cells.关键词
清肠化湿方/溃疡性结肠炎/过氧化物酶体增殖物激活受体-γ/p38丝裂原活化蛋白激酶/黏蛋白2/三叶因子3/大鼠Key words
ulcerative colitis/Qingchang Huashi decoction/peroxisome proliferator-activated receptor-γ/p38 mitogen activated protein kinases/mucins-2/trefoil factor-3/rat分类
医药卫生引用本文复制引用
顾培青,沈洪,朱磊,刘亚军,张露,刘军楼,徐艺,成家飞..清肠化湿方对TNBS大鼠结肠组织PPAR-γ/p38 MAPK的影响[J].山东中医药大学学报,2017,41(1):81-85,5.基金项目
国家自然科学基金委员会青年科学基金项目（编号81302919）；江苏省中医药局科技项目立项计划（编号LZ13048）；江苏省中医院高峰学术人才培养工程（编号k2014yrc13）；江苏省中医院院级课题 （）
