山东医药2017，Vol.57Issue(1)：1-3,3.DOI:10.3969/j.issn.1002-266X.2017.01.001
G 蛋白偶联胆汁酸受体1激活对高糖诱导小鼠心肌细胞肥大的影响
Effect of G-protein-coupled bile acid receptor 1 activation on high glucose-induced cardiomyocyte hypertrophy in mice
摘要
Abstract
Objective To investigate the effect of G-protein-coupled bile acid receptor 1 (TGR5)receptor activation on high glucose-induced cardiomyocyte hypertrophy in mice.Methods After primary culture of mouse cardiomyocytes,the cells were divided into the control group,high glucose group,high glucose +INT-777 (the agonist of TGR5 receptor)and high glucose +INT-777 +TGR5 shRNA group.Image analysis system was used for determination of cell surface area,RT-PCR and Western blotting were used to detect the lentivirus efficiency of TGR5 interference,and the expression of pro-hy-pertrophy genes was detected by RT-PCR.Results The mouse cardiomyocytes were successfully cultured.Compared with the control group,cardiomyocytes′surface area and pro-hypertrophy gene mRNA expression increased in the high glucose group (all P <0.05).TGR5 activation inhibited the increase of cardiomyocytes′surface area and pro-hypertrophy gene mRNA expression which was induced by high glucose (all P <0.05).Compared with high glucose +INT-777 group,car-diomyocytes′surface area and pro-hypertrophy gene mRNA expression increased significantly after TGR5 gene interference (all P <0.05).Conclusion TGR5 receptor activation can inhibit cardiomyocyte hypertrophy and pro-hypertrophy gene mRNA expression induced by high glucose,which may be an important drug target for diabetic cardiomyopathy.关键词
心肌细胞肥大/G蛋白偶联胆汁酸受体/高糖诱导/INT-777/心房钠尿因子/β-肌球蛋白重链/小鼠Key words
cardiomyocyte hypertrophy/G-protein-coupled bile acid receptor/high glucose induction/INT-777/atri-al natriure factor/β-myosin heavy chain/mice分类
医药卫生引用本文复制引用
冯健,吴丹,陈旭昕,刘应才,李家富..G 蛋白偶联胆汁酸受体1激活对高糖诱导小鼠心肌细胞肥大的影响[J].山东医药,2017,57(1):1-3,3.基金项目
国家自然科学基金资助项目（31300946）。 （）
