山东医药2017，Vol.57Issue(2)：10-13,4.DOI:10.3969/j.issn.1002-266X.2017.02.003
miR-214在舌鳞癌组织中的表达及其对癌细胞顺铂敏感性的影响
Expression of miR-214 in tongue squamous-cell carcinoma and its effects on cisplatin resistance
摘要
Abstract
Objective To explore the expression of miR-214 in tongue squamous-cell carcinoma (TSCC)and its effects on cisplatin resistance in tongue squamous-cell carcinoma cells,and to explore the underlying mechanism.Methods The expression level of miR-214 was detected by real-time PCR in 50 samples of TSCC tissues,20 samples of para-carci-noma tissues,10 samples of normal tongue mucosa tissues,TCA8113 /DDP cells and TCA8113 cells.The TCA8113 cells were transfected with miR-214 mimics (miR-214 mimics group)or mimics-NC (mimics-NC group),and the TCA8113 /DDP were transfected with miR-214 inhibitor (miR-214 inhibitor group)or inhibitor-NC (inhibitor-NC group).The ex-pression level of miR-214 was detected by real-time PCR,the half maximal inhibitory concentration (IC50 )value of DDP by CCK8 and the expression level of β-catenin and multidrug resistance (MDR)1 protein by Western blotting.Results The expression of miR-214 in the TSCC tissues was significantly higher than that in para-carcinoma tissues and normal tongue mucosa tissues (all P <0.05).The expression level of miR-214 in the TCA8113 /DDP cells was significantly higher than that in TCA8113 cells (P <0.05).After TCA8113 cells were transfected with miR-214 mimics,the IC50 value and expression levels of miR-214,β-catenin and MDR1 in the miR-214 mimics group were significantly higher than those in the mimics-NC group (all P <0.05).After TCA8113 /DDP cells were transfected with miR-214 inhibitor,the IC50 value and expression levels of miR-214,β-catenin and MDR1 in the miR-214 inhibitor group were significantly lower than those in the inhibitor-NC group (all P <0.05).Conclusion The expression of miR-214 is highly expressed in the tongue squa-mous-cell carcinoma,the down-regulation(up-regulation)of miR-214 can effectively increase (decrease)the sensitivity of tongue squamous-cell carcinoma cells to cisplatin,and this effect of miR-214 may be partly due to its regulation of Wnt/β-catenin signal pathway.关键词
舌肿瘤/舌鳞癌/微小 RNA-214/肿瘤耐药性/Wnt/β-catenin 信号通路/β-catenin 蛋白/多药耐药 1 蛋白Key words
tongue neoplasms/tongue squamous-cell carcinoma/mocro RNA-214/drug resistance of tumor/Wnt/β-catenin signal pathway/β-catenin protein/multidrug resistance 1 protein分类
医药卫生引用本文复制引用
唐海波,胡小华,刘敏..miR-214在舌鳞癌组织中的表达及其对癌细胞顺铂敏感性的影响[J].山东医药,2017,57(2):10-13,4.基金项目
四川省科技厅－泸州市科技局－泸州医学院联合项目（LY-51）；贵州省科教青年英才培养工程［黔省专合字（2012）192号］。 （）
