山东医药2017，Vol.57Issue(4)：21-24,4.DOI:10.3969/j.issn.1002-266X.2017.04.004
ADAM17-shRNA 对人乳腺癌 MCF-7细胞增殖的影响及机制
Effects of ADAM17-shRNA on proliferation of human breast cancer MCF-7 cells
摘要
Abstract
Objective To investigate the effect of a disintegrin and metalloprotease 17 ( ADAM17 )-shRNA on the proliferation of human breast cancer MCF-7 cells and to explore its possible mechanism .Methods MCF-7 cells were di-vided into the nonsense group , control group ( PBS) and transfection group .Four specific ADAM17-shRNAs ( ADAM17-shRNA-1219, ADAM17-shRNA-1508, ADAM17-shRNA-1134 and ADAM17-shRNA-297) were designed and synthesized for ADAM17 gene.MCF-7 cells were transfected by electroporation .The nonsense group was transfected by ADAM 17-shNC, the control group was added with PBS , and the transfection group was transfected by ADAM 17-shRNA-1219, AD-AM17-shRNA-1508, ADAM17-shRNA-1134 and ADAM17-shRNA-297, respectively.The expression of ADAM17 mRNA, the expression of ADAM17 protein, cell growth curve, proliferation activity and cell cycle of MCF-7 cells was respectively detected by real-time PCR, Western blotting, iCELLigence and flow cytometry .Results Four ADAM17-shRNA se-quences inhibited the expression of ADAM 17 gene, the shRNA1219 vector had the highest inhibitory efficiency , and com-pared with the nonsense group and control group , there was significant difference (all P<0.05).The expression level of ADAM17 mRNA and protein in the transfection group was significantly lower than that in the nonsense group and control group (all P<0.05).The cell proliferation activity and cell growth rate of the transfection group were significantly lower than those of the nonsense group and the control group (all P<0.05).The percentage of cells in S phase and G 2/M phase decreased in the transfection group , and most of the cells stayed in G0/G1 phase, which were significantly different from those in the control group and nonsense group (all P<0.05).Conclusion ADAM17-shRNA can inhibit the proliferation of human breast cancer MCF-7 cells, and the mechanism is related to its silencing effect on ADAM 17 gene.关键词
乳腺癌/解聚素-金属蛋白酶17/转染/RNA干扰/增殖Key words
breast carcinoma/a disintegrin and metalloprotease 17/transfection/RNA interference/proliferation分类
医药卫生引用本文复制引用
孟祥潮,蔡准,陈国福,吴丽君,张雪鹏..ADAM17-shRNA 对人乳腺癌 MCF-7细胞增殖的影响及机制[J].山东医药,2017,57(4):21-24,4.基金项目
河北省自然科学基金资助项目（C2010001767）；唐山市科学技术研究与发展计划项目［唐科计（2014）16号］。 （）
